This document describes the application of Agilent PLgel 10 µm MIXED-B columns in high molecular weight polymer analysis and demanding eluent conditions, such as polyvinyl pyrrolidone in dimethylformamide (DMF). Polyvinyl pyrrolidone is soluble in both polar organics and water. DMF is used with LiBr to minimize any polyelectrolyte effects. The document also highlights the features of PLgel MIXED-B columns, which are particularly useful for molecular weight distributions where slightly higher than average MWs are encountered.
Agilent Technologies presents an application note on analyzing Polyvinyl Pyrrolidone (PVP) dissolved in Dimethylformamide (DMF). The document highlights the suitability of Agilent PLgel 10 um MIXED-B columns for high molecular weight polymer analysis under demanding eluent conditions. It notes that PVP is soluble in polar organics and water, and DMF with Lithium Bromide (LiBr) is used to minimize polyelectrolyte effects.
| Column Particle Size | 10 um |
|---|---|
| Molecular Weight Range | up to 10 million |
| Flow Rate | 1.0 mL/min |
| Temperature | 60°C |
| Eluent Composition | DMF + 0.1% LiBr |
The columns are designed for analyzing polyvinyl pyrrolidone using dimethylformamide (DMF) as the eluent.
The PLgel MIXED-B columns span a wide range of molecular weights up to 10 million.
The recommended setup uses two columns with a length of 300 mm, an internal diameter of 7.5 mm, and a particle size of 10 µm.