This application note describes the use of the Polaris C18-A column to separate anti-inflammatory/analgesic drugs. The column can be used in ChromSep or Conventional mode, and a gradient solvent system of 45%-80% B is used with a flow rate of 1.0 ml/min. Detection is at 220 nm.
This document provides an application note for the Agilent 2217 LC system, specifically focusing on the separation of anti-inflammatory and analgesic drugs. It notes that Varian, Inc. is now part of Agilent Technologies and directs users to the Agilent website for more information.
The procedure utilizes a Polaris C18-A column (150x 4.6 mm, 5u) with two part numbers: A2000150C046 (ChromSep) and A2000150X046 (Conventional). The mobile phase consists of water with 0.1% formic acid (A) and acetonitrile with 0.1% formic acid (B), with a gradient of 45%-80% B over 10 minutes. The flow rate is set to 21.0 ml/min at ambient temperature, with detection at 1220 nm.
| Column | Polaris C18-A, 150x 4.6 mm, 5u |
|---|---|
| Part No. (ChromSep) | A2000150C046 |
| Part No. (Conventional) | A2000150X046 |
| Mobile Phase A | At HeO + 0.1% HCOOH |
| Mobile Phase B | CHsCN + 0.1% HCOOH |
| Gradient | 45%-80% B in 10 mins |
| Flow Rate | 21.0 ml/min |
| Temperature | Ambient |
| Detection | 1220 nm |
The application separates Aspirin, Carbamazepine, Salicylic acid, Tolmetin, Naproxen, Indomethacin, Diclofenac, and Ibuprofen.
The recommended column is Polaris C18-A, 150x 4.6 mm, 5u.
The detection is performed at 1220 nm.