This application note describes a procedure for the separation of four common analgesics by high performance liquid chromatography (HPLC) using a Polaris 5µ C8-Ether column. A 0.1% formic acid solution was used as the mobile phase. The four analgesics can be clearly seen in the chromatogram.
This document provides a liquid chromatography method for identifying specific analgesic compounds. The procedure utilizes an Agilent Polaris 5u C8-Ether column with dimensions of 150 x 4.6mm (Part Nr.: A2030150X046).
The analysis employs a gradient elution starting at 40% and increasing to 90% of solvent B over 10 minutes, with a flow rate of 21.0 ml/min. Detection is performed using UV detection at 220nm.
| Column Part Number | A2030150X046 |
|---|---|
| Column Dimensions | 150 x 4.6mm |
| Solvent A Composition | H2O + 0.1% HCOOH |
| Solvent B Composition | MeCN + 0.1% HCOOH |
| Gradient Program | 40% to 90%B in 10 min |
| Flow Rate | 21.0 ml/min |
| Detection Wavelength | UV @ 220nm |
The document lists acetaminophen, acetylsalicylic acid, salicylic acid, and ibuprofen.
The gradient elution runs for a total of 10 minutes.