This application note introduces an analytical method for the detection of hydrastine and berberine on Goldenseal using the Polaris 5µ C18-A, 150 x 4.6mm LC Column.
This document provides an application note for the analysis of Goldenseal. It specifies the use of a Polaris 5u C18-A column with dimensions of 150 x 4.6mm and part number A2000150X046.
The analysis utilizes a gradient elution starting at 20% and ending at 65% of solvent B (MeCN + 0.1% TFA) over 10 minutes, with a flow rate of 21.0 ml/min and a temperature of 135°C. Detection is performed using UV at 220nm, identifying the peaks as hydrastine and berberine.
| Column | Polaris 5u C18-A, 150 x 4.6mm |
|---|---|
| Part Number | A2000150X046 |
| Eluent A | H20 + 0.1% TFA |
| Eluent B | MeCN + 0.1% TFA |
| Gradient | 20 - 65%B in 10 min. |
| Flow Rate | 21.0 ml/min |
| Temperature | 135°C |
| Detection | UV @ 220nm |
| Identified Peaks | hydrastine, berberine |
The document specifies a Polaris 5u C18-A column with dimensions of 150 x 4.6mm.
The procedure requires a flow of 21.0 ml/min at a temperature of 135°C.
The peak identification list includes hydrastine and berberine.