This document describes the application of Tryptic Digest of bovine b-lactoglobulin A in 1998. The LC analysis was performed using a Polaris 5µ C18-A column with a gradient elution of H2O + 0.1% TFA and MeCN + 0.085% TFA. The digested products of bovine b-lactoglobulin A were analyzed using UV @ 215 nm detection.
This document is an application note from Agilent Technologies detailing the analysis of a tryptic digest of bovine b-lactoglobulin A. The text notes that Varian, Inc. is now part of Agilent Technologies.
The analysis was performed using an Agilent Polaris 5u C18-A column (Part No.: A2000250X046). The method utilizes a gradient elution from 15% to 45% B in 40 minutes with a flow rate of 21.0 ml/min and UV detection at 215 nm.
| Column | Polaris 5u C18-A, 250 x 4.6mm |
|---|---|
| Part Number | A2000250X046 |
| Eluent A | H20 + 0.1% TFA |
| Eluent B | MeCN + 0.085% TFA |
| Gradient | 15% to 45% B in 40 mins |
| Flow Rate | 21.0 ml/min |
| Detection | UV @ 215 nm |
The sample analyzed is a tryptic digest of bovine b-lactoglobulin A.
The part number for the column is A2000250X046.