This application note describes how to use an Agilent HPLC method to analyze imidazole compounds in caramel color.
This document is an Agilent Technologies application note (Application 1938) detailing the high-performance liquid chromatography (HPLC) method for analyzing imidazoles in caramel couler. The analysis utilizes a Polaris C18-A column with specific mobile phase conditions involving ammonium hydroxide and acetonitrile.
This document describes an HPLC method for analyzing imidazoles in caramel color.
The specified flow rate for the method is 0.4 ml/min.
The analysis must be performed at a temperature of 40°C.