Agilent Application Note 1936 HPLC Fatty Acids Analysis

This document presents an application of HPLC for fatty acid analysis. Using a Polaris™ 3µ C18-A column, the method successfully separates oleic acid, linoleic acid, and linolenic acid in under 52 seconds using an isocratic eluent. The method is efficient and fast.

Brand: Agilent
Document type: Product Leaflet
Language: English
Pages: 1
File format: PDF
File size: 31 KB
Published: 12 June, 2012
Updated: 28 September, 2023
MD5 checksum: 59AF7C580737351EA3E0D4C47C10D338
PDF: Agilent Application Note 1936 HPLC Fatty Acids Analysis PDF

About this manual

This document details an Agilent Technologies HPLC method for separating linolenic acid, linoleic acid, and oleic acid. The analysis utilizes a Polaris™ 3 C18-A column (30 x 4.6mm) with a flow rate of 1.5 mL/min.

What's inside

Fatty Acids
Column: Polaris 3 C18-A
Eluent Composition
Detection UV @ 205 nm
Separation Time

Frequently asked questions

What is the name of this application note?

The document is titled Application Note 1936.

How long does it take to separate three fatty acids using the described method?

Three 18-carbon fatty acids are separated in under 52 seconds.

What is the flow rate for the eluent used in this analysis?

The flow rate is set to 1.5 mL/min.

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