This document is about the use of Insulin Application 2004 LC Column
This document provides an application note for the analysis of insulin using Agilent Technologies equipment. It specifies the use of a Polaris 5u C18 column with dimensions of 150 x 4.6mm and part number A2000150X046.
The analysis utilizes a gradient elution method where the mobile phase consists of water with 0.1% trifluoroacetic acid (A) and acetonitrile with 0.1% trifluoroacetic acid (B). The gradient increases from 20% to 65% B over 10 minutes at a flow rate of 21.0 ml/min. Detection is performed using UV detection at 220nm.
| Column | Polaris 5u C18, 150 x 4.6mm |
|---|---|
| Part Number | A2000150X046 |
| Eluent A | H20 + 0.1% TFA |
| Eluent B | MeCN + 0.1% TFA |
| Gradient | 20-65% B in 10 min. |
| Flow Rate | 21.0 ml/min |
| Detection | UV @ 220nm |
The document analyzes bovine insulin and a bovine insulin impurity.
The flow rate is set to 21.0 ml/min.