This document provides a method for separating sulfonamide drugs using a Polaris 5µ C18-A, 150 x 4.6mm Part Nr.: A2000150X046 column
This document serves as an application note for the analysis of sulfa drugs using liquid chromatography. It specifies the use of the Agilent Polaris 5u C18-A column with dimensions of 150 x 4.6mm.
The note provides specific experimental parameters including the eluent composition (20mM K2HPO4, pH 9.0 mixed with MeCN 92:8), flow rate of 21.5 ml/min, and UV detection at 270nm. It identifies four specific peaks: sulfanilic acid, sulfamerazine, sulfisoxazole, and sulfapyridine.
| Column | Polaris 5u C18-A, 150 x 4.6mm |
|---|---|
| Eluent | 20MM K2HPO4, pH 9.0: MeCN 92:8 |
| Flow | 21.5 ml/min |
| Detection | UV @ 270nm |
The note identifies sulfanilic acid, sulfamerazine, sulfisoxazole, and sulfapyridine.
The document specifies using a Polaris 5u C18-A column with dimensions of 150 x 4.6mm.
The procedure requires an eluent of 20MM K2HPO4 at pH 9.0 mixed with MeCN in a ratio of 92:8.
The analysis uses a flow of 21.5 ml/min with UV detection set to 270nm.