This document is an application note for St. John’s Wort Application 2219 LC Column, which introduces the features and usage of the product
This document provides an application note for the Agilent 2219 LC system regarding the analysis of St. John's Wort. The procedure utilizes a Polaris C18 Ether 5p column with dimensions of 150 x 4.6 mm.
The analysis employs a mobile phase consisting of CH3CN and 25 mM KH2PO4 at a pH of 7.0 in a 40:60 ratio. The flow rate is set to 1.0 ml/min at ambient temperature, with detection performed via UV at 254 nm. The identified peaks include Uracil and Hypericin.
| Column | Polaris C18 Ether 5p, 150 x 4.6 mm |
|---|---|
| Part No. | A2020150X046 |
| Mobile Phase | CH3CN:25 mM KH2PO4, pH 7.0 - 40:60 |
| Flow Rate | 1.0 ml/min |
| Temperature | Ambient |
| Detection | UV, 254 nm |
The identified peaks are Uracil and Hypericin.
The flow rate is 1.0 ml/min.