This document describes the application of Sorbic acid and Salicylic acid on a Polaris Amide-C18 column, including the column dimensions, mobile phase composition, flow rate, temperature, and peak identification.
This document provides an application note for the Agilent 2234 LC system, detailing the separation of sorbic acid and salicylic acid. The procedure utilizes a Polaris Amide-C18 column with specific dimensions and a mobile phase consisting of water with 0.1% TFA and acetonitrile in a 50:50 ratio.
The analysis is performed at an ambient temperature with a flow rate of 1.0 mL/min and detection at 254 nm. The document lists several compounds identified in the analysis, including uracil, caffeine, sorbic acid, salicylic acid, and propyl paraben.
| Column | Polaris Amide-C18 |
|---|---|
| Dimensions | 150 x 4.6 mm, 5u |
| Part No. | A2006150x046 |
| Mobile Phase | H2O + 0.1% TFA:CH3CN - 50:50 |
| Flow Rate | 1.0 mL/min |
| Temperature | Ambient |
| Detection | 254 nm |
The separation uses a Polaris Amide-C18 column.
The column has dimensions of 150 x 4.6 mm.
The flow rate is set to 1.0 mL/min.