This document describes a method for separating artificial sweeteners on an Agilent Pursuit Diphenyl 5µ column.
This document is an application note from Agilent Technologies regarding the analysis of artificial sweeteners. It specifies the use of a Polaris 5u Amide LC column with dimensions of 150 x 4.6mm and part number A2006150X046. The note details the chromatographic conditions, including the eluent composition (Water + 0.1% Formic Acid at pH 2.0 mixed with Acetonitrile 94:6), flow rate of 21.0 ml/min, and UV detection at 254nm.
| Column | Polaris 5u Amide, 150 x 4.6mm |
|---|---|
| Part No. | A2006150X046 |
| Eluent A | H20 + 0.1% HCOOH, pH 2.0 |
| Eluent B | MeCN 94:6 |
| Flow Rate | 21.0 ml/min |
| Detection | UV @ 254nm |
The identified compounds include L-phenylalanine, beta-aspartyl L-phenylalanine, alpha-aspartyl L-phenylalanine, diketopiperazine, F-aspartyl phenylalanine, L-phenylalanine methyl ester HCI, beta-aspartame, and alpha-aspartame.
The application uses a Polaris 5u Amide column with dimensions of 150 x 4.6mm.
The eluent consists of water with 0.1% formic acid at pH 2.0 mixed with acetonitrile in a 94:6 ratio, flowing at 21.0 ml/min.
Detection is performed using UV at 254nm.