This is an application note about ß-Blockers Application 2326 LC technology
This document provides an HPLC method for the analysis of beta-blockers. The technique utilizes a Pursuit 5 C8 column (150 x 4.6mm) with a ChromSep Cartridge System. The analysis is performed at ambient temperature using a mobile phase consisting of MeCN and 0.1% NH4OH in water (pH 10.3) at a flow rate of 21.0 mL/min.
| Column | Pursuit 5 C8, 150 x 4.6mm |
|---|---|
| Part no. | Conventional A3030150X046 |
| System | ChromSep Cartridge System A3030150C046 |
| Temperature | Ambient |
| Eluent A | MeCN |
| Eluent B | 0.1% NH4OH in water, pH 10.3 |
| Ratio A:B | 65:35 |
| Flow rate | 21.0 mL/min |
| Detection | UV @ 215 nm |
The identified compounds are Hydrochlorothiazide, Practolol, Pindolol, Bisoprolol, and Alprenolol.
The specified flow rate is 21.0 mL/min.
Detection takes place at a UV wavelength of 215 nm.