Fatty Acids Application 2328 LC uses HPLC technology to analyze Pursuit™ 5µ C8, 150 x 4.6mm column and uses MeCN + 0.1% HCOOH and Water + 0.1% HCOOH as mobile phase, flow rate is 1.5 mL/min, detection wavelength is 205 nm, peaks are linolenic acid, linoleic acid and oleic acid.
This document provides an application note for analyzing fatty acids using High-Performance Liquid Chromatography (HPLC). It specifies the use of Agilent's Pursuit™ 5 C8 column within the ChromSep Cartridge System.
The procedure details specific chromatographic conditions, including a mobile phase consisting of acetonitrile and water with formic acid, an ambient temperature setting, and UV detection at 205 nm. The note identifies three key peaks: linolenic acid, linoleic acid, and oleic acid.
| Column Type | Pursuit™ 5 C8, 150 x 4.6mm |
|---|---|
| Part Number (Conventional) | A3030150X046 |
| Part Number (ChromSep Cartridge System) | A3030150C046 |
| Temperature | Ambient |
| Eluent A | MeCN + 0.1% HCOOH |
| Eluent B | Water + 0.1% HCOOH |
| Flow Rate | 1.5 mL/min |
| Detection Wavelength | UV @ 205 nm |
The procedure uses a Pursuit™ 5 C8 column with dimensions of 150 x 4.6mm.
The specified flow rate is 1.5 mL/min.