The document provides information about the features and characteristics of the E.coli Competent Cells BL21 (DE3) product, as well as instructions for use. This product is a type of competent cells that can be used for DNA chemical transformation. BL21 (DE3) strain is known for its high efficiency in expressing non-toxic proteins.
The manual describes the GeneCopoeia U0105A competent cells (BL21(DE3) strain) engineered for high‑efficiency T7‑driven protein expression. It lists the genotype, cell type, and typical transformation efficiency of the product.
It also gives detailed usage steps, from thawing on ice to heat‑shock, recovery in SOC medium, and plating, together with storage guidelines and safety notes for handling the cells. This manual is written in Chinese.
| Control DNA | pUC19, 10 pg/µl |
|---|---|
| Storage temperature | -80 °C |
| Storage duration | 12 months |
| Transformation efficiency | >1 × 10⁷ colonies per ng DNA |
Store the cells at –80 °C and keep them frozen for up to 12 months; do not refreeze after thawing.
Use less than 10 ng of plasmid DNA and keep the DNA solution volume under 20 µl to maintain high transformation efficiency.
Thaw cells on ice, mix with DNA, incubate on ice 30 min, heat‑shock 30‑60 s, recover in SOC at 37 °C for 1 h, plate on agar, and incubate overnight at 37 °C.