This manual describes the GeneCopoeia C0201A M‑MLV Reverse Transcriptase (RNase H‑) enzyme, its activity, storage conditions, and recommended reaction buffers. It explains how the enzyme synthesizes cDNA from RNA or DNA templates in the presence of primers and provides detailed step‑by‑step protocols for first‑strand synthesis.
Additional sections cover safety precautions, recommended primer concentrations, and a FAQ that addresses RNase H activity and template choices. The document also lists the composition of the 5× RT Reaction Buffer and storage buffer, and provides contact information for technical support. This manual is written in Japanese.
| Enzyme activity | 200 U/µl |
|---|---|
| Storage temperature | -20 °C |
| Storage buffer composition | 150 mM NaCl, 50 mM Tris‑HCl (pH 7.6), 0.1 mM EDTA, 1 mM DTT, 0.1 % NP‑40, glycerol |
| Reaction buffer (5× RT) | 25 mM each dNTP, 150 mM NaCl, 50 mM Tris‑HCl (pH 7.6), 0.1 mM EDTA, 1 mM DTT, 0.1 % NP‑40, glycerol |