E.coli Competent Cells 2T1 is a strain of E.coli that has a tetracycline resistance gene, high transformation efficiency, has removed mcrA, mrr, mcrBC, and hsdRMS restriction systems, tonA gene type can prevent phage T1 and T5 from infection, and the colony formation time is faster than other strains (12 hours)
The GeneCopoeia U0104A kit supplies E. coli 2‑T1 competent cells designed for cloning experiments. The strain carries tetracycline resistance and offers high transformation efficiency, making it suitable for constructing high‑quality genomic libraries.
The manual details storage at –80 °C for up to 12 months, thawing on ice, adding up to 10 ng DNA, a heat‑shock step at 42 °C for 30‑60 seconds, recovery in pre‑warmed SOC (or LB) medium, and plating for colony growth. This manual is written in Chinese.
| Transformation efficiency | >1 × 10^10 cfu/µg DNA |
|---|---|
| Competent cell volume per vial | 100 µl |
| Control DNA amount | 10 pg/µl |
Use 10 ng or less of plasmid DNA, keeping the DNA solution volume under 20 µl.
Heat‑shock at 42 °C for 30 to 60 seconds.
Yes, LB can replace SOC, but transformation efficiency may decrease.