The document describes the features and characteristics of the phi29 DNA Polymerase product kit, including efficient DNA synthesis, 3'→5' exonuclease proofreading activity, and special strand displacement and continuous synthesis characteristics.
This leaflet describes GeneCopoeia's phi29 DNA Polymerase (catalog number A0202A), an enzyme with high DNA synthesis capability and both 3'‑5' exonuclease and polymerase activities. It is supplied at a concentration of 100 (10 U/µl) and is intended for molecular biology applications such as whole‑genome amplification.
Key information includes storage at –20 °C, the composition of the 1× reaction buffer (50 mM Tris‑HCl pH 7.5, 10 mM MgCl₂, 10 mM (NH₄)₂SO₄, 4 mM DTT), a detailed storage buffer (100 mM KCl, 10 mM Tris‑HCl pH 7.4, 0.1 mM EDTA, 1 mM DTT, 0.596 % Tween 20, 0.596 % NP‑40, glycerol), and a step‑by‑step protocol for DNA amplification. This manual is written in Japanese.
| Enzyme concentration | 100 (10 U/µl) |
|---|---|
| 1× reaction buffer composition | 50 mM Tris‑HCl pH 7.5, 10 mM MgCl₂, 10 mM (NH₄)₂SO₄, 4 mM DTT |
| Storage buffer composition | 100 mM KCl, 10 mM Tris‑HCl pH 7.4, 0.1 mM EDTA, 1 mM DTT, 0.596 % Tween 20, 0.596 % NP‑40, glycerol |
| Recommended storage temperature | -20 °C |
Store the enzyme at –20 °C.
The 1× buffer contains 50 mM Tris‑HCl (pH 7.5), 10 mM MgCl₂, 10 mM (NH₄)₂SO₄, and 4 mM DTT.
The enzyme activity is 10 U/µl (100 units per ml).