The First Strand cDNA Synthesis Kit is a reverse transcription kit launched by GeneCopoeia Inc. It is used to synthesize single-stranded cDNA for gene cloning and RNA research. The kit contains all the reagents required for the reaction. Users only need to provide RNA samples to perform the reverse transcription experiment.
The First Strand cDNA Synthesis Kit (product code C0210A) from GeneCopoeia uses Moloney Murine Leukemia Virus reverse transcriptase (M‑MLV RTase, RNase H‑) to synthesize single‑strand cDNA from total RNA or poly(A) mRNA. All necessary reagents, including 5× RT Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, and Sequence‑Specific Primer, are supplied in a single kit.
The manual outlines storage conditions, handling precautions, and a complete workflow: preparation of the RNA‑Primer mix, reverse transcription (37 °C or 42 °C for 1 hour depending on primer), enzyme inactivation at 85 °C, and downstream PCR or real‑time PCR usage. An experimental example demonstrates how to verify reverse‑transcription efficiency by PCR amplification of GAPDH. This manual is written in Chinese.
| Storage temperature | -20°C |
|---|---|
| Reaction total volume | 13 µl |
| Enzyme | M‑MLV Reverse Transcriptase (RNase H‑) |
| 5× RT Reaction Buffer | Included |
| RNase Inhibitor concentration | 25 U/µl |
The kit should be stored at –20 °C.
Incubate at 42 °C for 1 hour.
Heat the reaction at 85 °C for 5 minutes, then store at –20 °C.
Yes, the cDNA can be added directly to a PCR mix without purification.