2×AllinOne TM Q-PCR Mix ROX +is a special reagent for fluorescence quantitative PCR (Real-Time PCR), which contains DNA polymerase, reaction buffer, dNTP, SYBR Green I and ROX. When using, just add template and primers to perform Real-Time PCR experiment on ABI instrument, the operation is very convenient and quick.
This manual describes the 2×AllinOne Q‑PCR Mix ROX+ reagent, which contains DNA polymerase, buffer, dNTPs, SYBR Green I and ROX for quantitative real‑time PCR on ABI platforms. The product is supplied as two 1 ml tubes (20 µl reaction ×200 times) and is stored at –20 °C.
The document includes detailed operating instructions, recommended primer concentrations, PCR cycling parameters, data analysis guidance, an experimental example, and a troubleshooting section that addresses common issues such as noisy amplification curves and poor reproducibility. This manual is written in Chinese.
| Storage temperature | -20 °C |
|---|---|
| Packaging | 1 ml × 2 (20 µl reaction × 200 times) |
| Final primer concentration | 0.4 µM |
Store the 2×AllinOne Q‑PCR Mix at –20 °C and protect it from light.
A typical primer concentration is 0.4 µM, with a usable range of 0.2 µM to 0.6 µM.
Check and adjust the fluorescence detection temperature, verify sample positions, and consider modifying primer concentration or annealing temperature.