This document introduces the Agilent 2100 bioanalyzer and LabChip DNA analysis technology, which has the advantages of high speed, high precision, and ease of use
The document introduces the Agilent 2100 bioanalyzer and its use with DNA 500 and DNA 1000 LabChip kits for high-resolution DNA analysis. It highlights advantages over traditional slab gel electrophoresis, including increased speed, improved data precision, and standardized conditions that yield reproducible results.
| Size range (DNA 500) | 25 to 500 base pairs |
|---|---|
| Size range (DNA 1000) | 25 to 1000 base pairs |
| Resolution (DNA 500, 25-100 bp) | 5 base pairs |
| Resolution (DNA 500, 100-500 bp) | 5 % |
| Resolution (DNA 1000, 500-1000 bp) | 10 % |
They are especially useful for small to medium sized PCR and RT-PCR products, can resolve primer dimers, and apply to competitive PCR and cleavage based applications such as RFLP.
The resolution in the lower range, i.e. below 100 bp, is significantly increased compared to other DNA LabChip kits.
The maximum error for quantitation remains at 30 % (this corresponds to a 95 % confidence level).