Agilent Manuals

High-Speed Separation of Water-Soluble Vitamins with Ion-Pairing Chromatography

This document introduces a high-efficiency and high-speed separation method for natural anthocyanins. The method uses ZORBAX StableBond SB-C18 columns and phosphoric acid mobile phase, which can achieve high-efficiency separation of more than 25 anthocyanins.

This document introduces the results of an experiment on using ZORBAX Sil for high-performance liquid chromatography separation of vitamin A isomers (retinal isomers).

This document discusses the effect of mobile-phase solvent strength in the separation of mono- and disaccharides. Good resolution of various sugars can be achieved using a ZORBAX NH2 column. The retention of sugars increases with increasing organic content in the mobile phase.

This application demonstrates a reversed-phase separation using high-organic concentration (>90% methanol) on a ZORBAX XDB-C8 column. By using reversed-phase chromatography for water-soluble and fat-soluble vitamins, labs requiring both assays can now minimize changeover time.

This document introduces the characteristics of ZORBAX 300SB-C8 and 300SB-CN two chromatographic columns from Agilent Technologies, which can achieve stable separation of high molecular weight, alkylated, wheat glutenin protein subunits.

This document describes a method for detecting EGCG in tea leaves. The method uses the Agilent ZORBAX SB-C8 column to separate a group of catechins in tea leaves under low pH conditions, and achieves good separation.

This document describes a high-speed HPLC method for separating parabens. The method uses a ZORBAX StableBond SB-C18 column and 0.1% H3PO4:ACN as the mobile phase. The run conditions are 2 mL/min flow rate, 1 µL injection volume, and 60℃ column temperature. The method can separate parabens in less than one minute.

This document introduces the application of Agilent 1100 quaternary pump equipped with ZORBAX Eclipse-XDB column in food analysis. The column has good chemical stability, and can be used for chromatography separation at intermediate pH values. By shortening the column length and particle size, the analysis time can be shortened, the separation can be maintained, and the solvent consumption can be reduced.

This document introduces the method for analyzing various flavoring agents in food using Agilent ZORBAX SB-Phenyl, Narrow-Bore LC/MS column

The application note introduces Agilent ZORBAX Eclipse XDB-Phenyl column for flavor and food analysis, which has excellent separation ability and good peak shape.

Agilent ZORBAX NH2 column has excellent resolution and retention in high performance carbohydrate analysis

This document describes the method of separating vitamins D2 and D3 using the Agilent ZORBAX ODS column. The results show that the non-endcapped column has better retention and resolution than the endcapped column.

Aspartame is an artificial sweetener used as a sugar substitute in diet drinks and foodstuffs. This document introduces the analysis method of aspartame and its metabolites in food.

This document describes the separation performance of Agilent ZORBAX 300 SB-C8 for complex plant protein samples, and the effect of temperature in reverse phase separations of complex samples.