Agilent Manuals

Agilent 6410 Triple Quadrupole Mass Spectrometer (QQQ) is used to analyze several water-soluble B-vitamin compounds in breakfast cereal, including thiamine, riboflavin, pyridoxine, vitamin B12, pantothenic acid, biotin, and niacinamide, which are present at relatively high levels. A simple gradient elution is carried out on a Rapid Resolution High Throughput SB-Aq column (particle size 1.8 µm). All compounds elute in less than 7.5 minutes, and with the exception of pyridoxine, good linearity over more than three orders of magnitude, from 0.5 to 500 ppb, is demonstrated with good peak area reproducibility at the 0.5 ppb level, which is the lowest level of quantitation considered.

The document introduces a method for authenticating basmati rice using the Agilent 2100 bioanalyzer and the DNA 1000 assay. This method is quick, cost-effective, and can be used to estimate the level of non-basmati rice in ground rice products.

This document describes a novel approach to studying the production of secondary metabolites by fungi using LC/TOF-MS. Fungi grown on culture media are solvent-extracted and directly analyzed by LC/TOF-MS, allowing for easy identification of 465 secondary metabolites, mycotoxins, and other compounds of interest in a database. The methodology was validated by spiking culture media with 20 mycotoxin standards and identifying these toxins in the crude solvent extracts. Subsequently, using seven different fungi from culture collections, after culturing for 7 to 14 days in three different media, anticipated metabolites were readily identified.

Analyzing a complex matrix can be accomplished using multiple specific detectors, but a significant time savings is realized using GC/MSD. Using an available Retention Time Locked database with Deconvolution Reporting Software (DRS) adds a second “expert” opinion. GC/MSD with DRS provides the fastest methodology to the fewest number of false positives/negatives with the greatest confidence in the results.

Analysis of Suspected Flavor and Fragrance Allergens in Cosmetics Using the 7890A GC and Capillary Column Backflush Application

This paper describes a new methodology for amino acid analysis using recently developed columns with an engineered particle size of 1.8 µm. These patented particles are specifically designed to create less backpressure than other sub-two-micron particles on the market; therefore, they can be used on 400 bar HPLC systems as well as higher pressure limit instruments.

A Deans switch, employing Agilent's Capillary Flow Technology, was configured on an Agilent 7890A gas chromatograph (GC) equipped with dual electron capture detectors (ECDs). A method was developed for the analysis of fish oil for polychlorinated biphenyl (PCB) contamination. The Deans switch was used to heart cut 7 indicator PCBs (IUPAC congeners 28, 52, 101, 118, 138, 153, and 180) from the primary DB-XLB column onto a DB-200 column for further separation. Fish oil from a supplement capsule was simply diluted 1:10 in isooctane and injected directly. In a separate experiment, the fish oil was analyzed by GC with a flame ionization detector (GC/FID) without backflushing. From these analyses, it was estimated that about two-thirds of the fish oil components would remain on the column after the 17.4-minute GC/ECD run. To prevent carryover, contamination, and retention time shifts, the Deans switch was used to backflush the primary column at the end of each run. Evidence shows that backflushing removed the fish oil residue, which otherwise would quickly degrade the chromatography.

This document describes a method for improving productivity and extending column life using backflush application. The method includes using a 3-way splitter to divide the column outlet into two branches, each of which is connected to a GC detector, as well as an MSD. This configuration provides full-scan data for library searching, SIM data for quantitation, and element selective detector data for excellent selectivity and sensitivity from complex matrices.

This article describes the use of RTL and a three-way splitter to identify unknowns in strawberry extract.

This document describes a method for detecting chloramphenicol in honey, shrimp, and chicken using the Agilent G6410AA Triple Quadrupole Mass Spectrometer (QQQ). The sensitivity obtained exceeds the minimum required performance level (MRPL) established by the European Union regulation for food monitoring programs. Using a deuterated internal standard and one simple sample solid phase extraction (SPE) procedure can provide a limit of detection at 10 ppt in sample matrix. The analytical performance of the method was evaluated for three different matrixes and the results show little or no matrix effects. Linearity of response over 2 orders of magnitude was demonstrated (r > 0.99). In addition, good reproducibility of the two required product ion ratios was obtained to meet the EU identification points needed for confirmation. This study is a valuable indicator of the ability of the QQQ for routine quantitative trace analysis of chloramphenicol in honey, shrimp, and chicken.

This article illustrates how time-of-flight mass spectrometry can be used in the safety evaluation of new and existing can coatings used in the food industry. The accurate mass provides information for the parent compound and fragment ions greatly increase the confidence in the identification process.

An improved analytical method that offers rapid, accurate determination and identification of 22 amino acids in a variety of matrixes is reported. The amino acids were extracted from the matrixes using acidified water. Simul- taneous determination of 22 underivatized amino acids was carried out by liquid chromatography–mass spec- trometry (LC/MS). A narrow-bore column allowed rapid screening and quantitative analysis by LC/atmospheric pressure chemical ionization (APCI) MS in positive ion mode.

The analytical method uses LC/MS/MS to detect 4 banned nitrofuran antibiotics metabolites, furazolidone, furaltadone, nitrofurantoin and nitrofurazone in fish, and the method is highly sensitive, down to 0.01 ppb (10 ppt). The method meets all the confirmation criteria set by the European Union for analytical method performance. The method is suitable for the analysis of fish and other aquaculture samples.

This application note describes a complete method to rapidly and precisely determine residue levels of malachite green and leucomalachite green in fish with the new Agilent 6410 LC/MS triple quadrupole system. Using positive mode electrospray ionization (ESI+) and multiple reaction monitoring (MRM), qualification and quantification were accomplished without the traditional tedious PbO2 oxidation process. The LC/MS/MS method’s LOQ is 0.01 µg/Kg, which easily meets the import requirement of 2 µg/Kg set by Japan and the EU.

This study used Agilent G6410AA triple quadrupole mass spectrometer (QQQ) to detect 100 pesticides residues in vegetables and fruits