Agilent Manuals

This Application Note compares the results of the development of an accurate and reproducible method to analyze paracetamol and related impurities according to European Pharmacopoeia (EP) and USP regulations1, using an Agilent 1120 Compact LC and a 1200 Series LC system.

This Application Note describes the analysis of several degradation products of metoprolol with the Agilent 1120 Compact LC. The preceding method development was done with the Agilent 1200 Series Rapid Resolution LC (RRLC) system. This Application Note starts with the final result of method development, to prove that no further development is necessary. It shows that the Agilent 1120 Compact LC works as a reliable and highly robust instrument for standard LC methodology. The transfer from rapid resolution separation material to conventional columns by the Method Translator Software is shown. The results of system suitability and performance tests prove the reliability and quality of the Agilent 1120 Compact LC system for everyday quality control testing.

This Application Note describes the analysis of several well known preservatives like benzoic acid, sorbic acid or the esters p-hydroxy benzoic acid (methyl-, ethyl- and propyl-) with the Agilent 1120 Compact LC. It demonstrates the relevance of this method for quality control testing and monitoring of products for consumer protection.

This Application Note describes the development and validation of a method for simultaneous determination of paracetamol, diclofenac and ibuprofen using the Agilent 1120 Compact LC. The method has excellent precision, accuracy and low limits of detection.

This application note describes an analytical method for the determination of herbicides in drinking water using the Agilent 1120 Compact LC. The method is characterized by high retention time and peak area precision and low detection limits.

This Application Note describes the use of the Agilent 1120 Compact LC to analyze antioxidants in chewing gum.

Using the Agilent 1200 Series Rapid Resolution LC system and Agilent Method Translator software, USP pravastatin sodium chromatographic purity method is used to conduct a fast LC analysis. This application note describes how to start from a USP method, incorporate the allowed adjustments, and produce a fast method that meets system suitability requirements.

This application note describes the method for generating non-linear ELSD calibration curves using ChemStation software and the quantification of non-UV active compound topiramate using this curve.

This Application Note demonstrates efficient methods for conserving acetonitrile using Agilent's ZORBAX columns as a solution to the global acetonitrile shortage.

This document describes how to accelerate the entire method development process by using Agilent Rapid Resolution HT columns in combination with the Agilent 1200 Series Rapid Resolution LC (RRLC) system.

These single, dual and quad channel, hermetically sealed optocouplers provide military-grade reliability and high performance in a wide variety of harsh industrial applications.

This Application Note describes the isolation of three antianginal drugs (nifedipine, nisoldipine, and nimodipine) from a mixture as an example application for purification of pharmaceutical drugs using the Agilent 1100 Series purification1,2 system PS (preparative scale). The goal of the application was to purify 20 mg of each compound in a single run and to get three fractions containing only the desired substances.

This Application Note describes a method for generating a small method set based on retention time windows in the pre-preparative analytical run. Using Agilent standards with known elution properties makes this an easy four-step process, which can be accomplished in less than one hour. An application example demonstrating how the resolution of the target compound can be improved is also shown.

This article discusses the importance of impurity isolation and purification in impurity profiling using the Agilent 1200 Series LC system, and elucidates the structures of impurities through NMR analysis. By optimizing the method and conducting loading experiments, the impurities were successfully isolated from the crude sample with high purity and recovery. Sufficient amounts of each compound were obtained through repetitive injections for further structural elucidation.