Agilent Manuals

Using ZORBAX Eclipse XDB-C18 Rapid-Resolution (3.5µm) Cartridge-Column, can simultaneously reduce column length and particle size, thereby reducing analysis time, maintaining separation, and reducing solvent usage.

This document describes the use of a 3mm diameter ZORBAX Low-Volume Column for analysis of pesticides in drinking water. 28 pesticides are separated with good resolution and peak shape in a single run using simple mobile phases.

This document presents examples of useful differences in band spacing that can be obtained by varying column type while keeping mobile phase conditions constant. The stationary phase strength (retentivity) is in the order C8>Phenyl>CN for these sterically protected bonded phases. Long-term stability and reproducibility are provided by ZORBAX StableBond columns. A wide range of herbicides can be separated with excellent peak efficiency and peak shape.

This document introduces the use of Agilent ZORBAX 3.5 µm particles in HPLC rapid gradient separation of plant hormones.

This research used gas chromatography/mass spectrometry (GC/MS) and electrospray ionization liquid chromatography/mass spectrometry (ESI-LC/MS) to analyze a commercially available fungicide formulation. GC/MS analysis provided a detailed look at the volatile components in the formulation, but did not yield any results for the active ingredient, triforine. ESI-LC/MS provided information on the stereoisomers of triforine as well as the nonvolatile surfactants and contaminants in the formulation. This paper demonstrates the complementary nature of these two analytical techniques when trying to fully characterize a complex chemical formulation containing a broad range of components.

This application note describes two retention time locked methods and retention time databases that improve the analysis of fatty acid methyl esters. In the first method, fatty acid methyl esters are analyzed on a DB-Wax column using gas chromatography/flame ionization detector or gas chromatography/mass spectrometry. In the second method, a DB-23 column is used. Retention time and mass spectral libraries are available for both methods. Retention time locking allows easy peak identification, easy exchange of data between instruments (gas chromatography/flame ionization detector, gas chromatography/mass spectrometry, different labs), and avoids the need to modify the retention times in calibra- tion tables after column maintenance or column change.

Rapid Analysis of CLP Pesticides Using High-Temperature DB-35ms and DB-XLB Columns

This note describes the results of using hydrogen carrier gas and increasing the oven ramp rate in the analysis of chlorinated pesticides and herbicides using DB-35ms and DB-XLB columns.

Methods for detecting genetically modified organisms (GMOs)

This document describes the use of liquid chromatography/mass selective detection (LC/MSD) technique to replace the nonspecific post-column derivatization process for detecting low ppb levels of carbamate pesticides in food. System linearity, sensitivity, and repeatability were evaluated on nine carbamates, and the instrument detection limits were determined to be about 3 to 10 ppb for all the analytes in broccoli extract. This method overcomes the false positive issue of fluorescence detection method, providing higher specificity, no additional hardware and plumbing requirements, and lower limits of detection.

This application note describes the use of the Agilent 7500c ICP-MS system to measure major, minor and trace elements in two standard reference plant materials.

This document describes a method for quantifying pesticides in the parts-per-trillion range using retention time locking and GC/MS application. By using a selected ion monitoring method and large volume injection capability, the target compound quantitation limit can be lowered to the low parts-per-billion (pg/µL) range. Additionally, an automated 567-compound retention time locking pesticide mass spectral library can screen an acquired sample's data file for all 567 compounds in seconds. The library can also be used for rapid screening of samples acquired in selected ion monitoring method. Using the compound library information, a selected ion monitoring method for 80 target compounds was created in less than 2 hours without running any analyses.

This document introduces the method for the detection of 3-chloro-1,2-propanediol (3-MCPD) in hydrolyzed vegetable protein.

Sugars and sugar alcohols were successfully analyzed by negative ion mode of liquid chromatography/mass spectrometry using atmospheric pressure chemical ionization

This paper describes a method for analyzing triglycerides using liquid chromatography/mass spectrometry. By employing atmospheric pressure chemical ionization in positive ion mode, the composition of triglycerides can be analyzed quickly and accurately. This method requires simple sample preprocessing without saponification and offers high sensitivity and separation capacity.