Agilent Manuals

This Application Note describes a more sophisticated approach to peak-based fraction collection by using the parameters up and down slope, upper threshold and maximum peak duration.

To increase throughput in preparative HPLC, it is necessary to load high amounts of sample onto the column. This application note describes two common techniques - sandwich injection and organic-phase injection, and how they can be performed easily on an Agilent 1100 Series purification system.

This document describes the application of the Agilent 1100 Series 12-position/13-port valve in compound isolation and purification in the pharmaceutical industry. The system is optimized for high throughput, capacity, and productivity. This application note demonstrates how the Agilent 1100 Series valve can enhance the purification system by adding easy recovery collection and simple time-based fraction collection.

This application note demonstrates the configuration of the Agilent 1200 Series RRLC AS purification system and the use of an optimized method for purifying dye-labeled or Fmoc-protected peptide nucleic acids from solid-phase synthesis. It provides an overview of the characteristics and applications of peptide nucleic acids.

This Application Note describes a method of injecting large sample volumes using an Agilent 1100 Series purification system and injection pump.

This document introduces the Agilent 1200 Series LC Method Development Solution and Agilent Easy Access software LC walk-up system, which can be used for reaction kinetics, column scouting, and impurity checks with up to eight columns

Fast LC methods can help to increase sample throughput significantly without compromising data quality.

The Agilent 1290 Infinity Multi-method Solution for LC allows analysis of seven different food applications on one instrument without column or mobile phase changes. It features automated switching between columns, solvent selection, and the ability to configure different separation conditions.

Detection and identification of impurities in pharmaceutical drugs using Agilent MassHunter software

4-Plex iTRAQ Based Quantitative Proteomic Analysis Using an Agilent Accurate -Mass Q-TOF

This Application Note describes a fast quality control method for the analysis of notoginsenoside R1 and Ginsenosides Rg1, Re and Rb1 using the Agilent 1290 Infinity LC system and an Agilent ZORBAX RRHD 1.8 µm column. Compared to conventional methods, the rapid method is much faster, and maintains the same performance and quality of separation. In addition, solvent consumption is also dramatically reduced.

A method was developed using ultra high performance liquid chromatography coupled to triple quadrupole mass spectrometry (LC/MS/MS) to quantitatively determine alprazolam in dried blood spots (DBS).

This application note describes a method for determining the N-nitrosamine content in rubber articles using the Agilent 7000A triple quadrupole GC/MS system. The method employs an internal standard, N-nitroso-N-dipropylamine (NDPA), for the trace-level detection and confirmation of seven target N-amines, N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosodibutylamine (NDBA), N-nitrosopiperidine (NPIP), N-nitrosopyrrolidine (NPYR), N-nitrosomorpholine (NMOR), and N-nitroso-N-ethyl-N-phenylamine (NEPhA). The resulting low method detection limit, good linearity, and sample recovery demonstrate the high selectivity and sensitivity of the method described in this application note for trace-level detection and confirmation of the targeted N-amines in complex sample matrices.

This document discusses the important impact of RNA quality on miRNA expression analysis, as well as the application of the Agilent 2100 Bioanalyzer and Agilent Small RNA Assay in RNA integrity assessment and miRNA analysis.