Agilent Manuals

A sensitive and selective analytical method for the determination of 44 pesticide residues in several foodstuffs using the Agilent G6410AA Triple Quadrupole Mass Spec- trometer (QQQ) was developed. This method use two dif- ferent sample preparation methods followed by LC/MS/MS (liquid chromatography/tandem mass spec- trometry). The limits of detection for all pesticides were less than 10 ng/mL in foodstuff. The sensitivity of QQQ easily met the maximum residue limits (MRLs) of all investigated pesticides in Japan Food Hygiene Law.

This paper introduces a rapid LC/MS method for determining hydroxymethylfurfural in foods. The method has high sensitivity, selectivity, and a low detection limit

The increased availability of sub-2-micron (STM) columns and increased demand for methods friendly to mass spectrometers has led to a strong trend toward conversion of existing HPLC methods to smaller diameter and smaller particle size columns. While the conversion is a simple mathematical exercise requiring the scaling flow rates, gradient times, and injection volumes, many users observe less than perfect results. Here we look closely at the problem and propose calculations that improve the speed and/or resolution in a more predictable and beneficial way.

This application describes how to use automated online trace enrichment (SPE) cartridges and a rapid switching valve in a Rapid Resolution LC (RRLC) system for rapid analysis of chlorinated phenoxy acid herbicides. Using small 3.5- and 1.8-µm ZORBAX RRHT columns and an automated online SPE cartridge in place of the loading pump specified in the EPA method dramatically reduces the total analysis time and virtually eliminates the potential for sample cross-contamination.

This document introduces an updated and expanded mass spectral library for analysis of pesticides and endocrine disruptors. The library contains 926 pesticides, endocrine disruptors, and related compounds, 359 more than the original library. All compounds have locked retention times that can be accurately reproduced using an Agilent GC/MS system with the ChemStation's Retention Time Locking software. The document also explains the use of Agilent's Screener software and Deconvolution Reporting Software for compound identification and screening.

Using licorice, a licorice hydrolysis product, and commercial licorice samples, high-resolution reversed-phase HPLC analytical studies showed that resolution and throughput using a ZORBAX 1.8-µm column greatly exceeded that obtained using the conventional 5.0-µm column.

This document demonstrates the effective interference removal of a range of elements in a challenging and complex sample matrix using the Agilent 7500ce ICP-MS with a single set of He cell gas conditions, highlighting its high performance.

This document is about a high throughput HPLC method for separating xanthines. The method uses a simple isocratic HPLC method and can be used to analyze theobromine, theophylline and caffeine in liquid refreshments (tea, chocolate syrup, and cocoa).

This application note presents the capability of the Agilent Time of Flight Mass Spectrometer (LC/MSD TOF) to be used as a screening, confirmation and quantitation tool-within-one analytical run of 5 min.

This article introduces the analysis method of fatty acid methyl esters (FAMEs), including the separation using different stationary phases. It is found that polyethylene glycol columns are suitable for the separation of simple samples, while medium polar cyanopropyl columns (DB23) are suitable for the separation of complex FAME mixtures and achieve some cis-trans separation. The highly polar HP-88 cyanopropyl column is preferred for more detailed cis-trans separation.

This document describes a rapid method for the analysis of paraben preservatives using reversed-phase HPLC.

The use of liquid chromatography/ion trap mass spectrometry (LC/ITMS) and liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) for the identification and quantitation of ter- buthylazine in olive oil samples

This application note details how the Agilent 2100 Bioanalyzer was used in polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) fragment analysis for fish species identification. A 464bp cytochrome-b target sequence, found in all vertebrate fish, was first amplified and then digested with restriction enzymes. The fragments were resolved on the DNA 500 LabChip®, allowing simple comparison with authentic species profiles due to the accuracy of fragment size determination. Use of the Bioanalyzer offered significant benefits over traditional gel electrophoresis and DNA staining techniques for PCR-RFLP fragment analysis.

This paper describes the configuration and operation of a combined liquid chromatography/mass spectrometry (LC/MS) system for the identification and isolation of DSP-toxins. In the analytical mode, okadaic acid (OA) and dinophysistoxin-1 (DTX-1) are more selectively and sensitively monitored when compared to LC with fluorescence detection. With less sample preparation, the detection limits are decreased by a factor of 3–5, depending on the matrix. In semipreparative mode, OA and DTX-1 could be isolated from crude extracts of Prorocentrum lima algae using mass-based fraction collection with a purity >98%. Due to this method, reference standards of DSP toxins are now commercially available.