Agilent Manuals

This document introduces the application of Agilent RapidFire/MS systems for high-throughput in vitro ADME analysis, with a focus on cytochrome P450 (CYP) inhibition. The system combines high-throughput sample processing with triple quadrupole (QQQ) or time-of-flight (TOF) mass spectrometry (MS) to streamline ADME assay analysis.

This document describes a method for analyzing antibiotics in meat using an Agilent Poroshell 120 EC-C18 column. The method uses a gradient method and UV detection, and it can be quickly optimized.

Agilent Technologies provided an application compendium that introduces detailed methods for the analysis of rubbers by GPC/SEC, including natural rubber, synthetic rubber, polydimethyl siloxane, etc.

This document introduces the solutions provided by Agilent Technologies in the food and agriculture field, including products, applications, software and services

This document describes the application of Agilent RapidFire/MS systems for high-throughput in vitro ADME analysis. Permeability assays such as Caco-2 and PAMPA are used to mimic the absorption of potential drug candidates by human intestinal cells. The Agilent RapidFire High-throughput Mass Spectrometry System, coupled with QQQ or TOF MS, enables rapid sample processing and provides results comparable to traditional LC/MS methods in a fraction of the time, making it a cost-effective and highly productive method for assessing permeability in the drug discovery process.

This Application Note describes the analysis of free and total glycerol in B-100 biodiesel methyl esters using the Agilent Select Biodiesel for Glycerides metal capillary (UltiMetal) GC column in high temperature conditions.

This article discusses the widespread use of vanilla extract as a flavoring ingredient in foods and beverages. The main compounds in vanilla extract are vanillin, 4-hydroxybenzaldehyde, vanillic acid, and 4-hydroxybenzoic acid. Due to limited availability and high prices of natural vanilla extract, artificial vanilla flavors are often used. It is important to differentiate between natural and artificial vanilla flavors. The article presents a conventional HPLC method and a UHPLC method for analyzing various natural and artificial vanilla flavors.

This application note describes the optimization of the 1H-15N CIGAR experiment for long-range nitrogen detection using VnmrJ 3 software. The software provides easy-to-use, interactive tools for setting up advanced experiments, allowing even novice users to obtain critical information about their research samples using the most advanced NMR experiments available.

This document presents the LC/MS/MS determination method for vitamin D in food. It describes the importance of vitamin D in regulating calcium and phosphate levels in the body and promoting healthy bone development. It also discusses the sources of vitamin D, including sunlight exposure and various food sources.

This Application Note discusses the concept of dried matrix spot homogeneity and demonstrates the homogeneity of blood spots on an Agilent Bond Elut DMS card. Results show that the size of the blood spots and the analyte dispersion is homogeneous, and the recoveries are reproducible regardless of whether the sample is taken from the center or the edge of the blood spot.

The method developed in this work was based on immunoaffinity clean-up and electrochemical derivatization. The method showed good linearity (0.1-10 µg/L), sensitivity (LOD as low as 0.004-0.007 µg/L), and precision (RSDs less than 0.1% and 0.3%). It can be used for the simultaneous determination of four aflatoxins in corn and peanut butter.

The document introduces an economical instrument configuration using the Agilent 7820A GC and 7697A headspace sampler for measuring ethanol in blood. It has good repeatability and easy-to-use operation, making it ideal for routine QA/QC labs. According to national standards, drunk driving is defined as a concentration of ethanol in the blood of 80 mg/100 mL or more, while drinking and driving is defined as a concentration of ethanol in the blood of 20 mg/100 mL or more. To meet this quantitative requirement, the calibration curve for the method was generated in a wide range of 10 mg/100 mL to 160 mg/100 mL, allowing both limits to be identified.

This Application Note demonstrates the use of the Agilent 2100 Bioanalyzer and a dedicated Plant RNA assay to assess the integrity of plant RNA samples from multiple plant sources and differing degradative states.

Analysis of Carcinogenic Tobacco- Specific Nitrosamines in Mainstream Cigarette Smoke Using an Agilent J&W DB-35ms Ultra Inert GC Column