Agilent Manuals

This Application Note shows three system configurations for recovery collection with the Agilent 1100 Series purification system1,2. One configuration uses a 12-position/13-port valve 3 in the waste line of the fraction collector, which offers up to twelve recovery locations. Another possibility is to use a third-party fraction collector, controlled by the ChemStation through external contacts using a BCD board. The most sophisticated approach, however, is to use a dedicated Agilent 1100 Series fraction collector, which gives complete recovery location tracking with the graphical user interface of the software or in the reports.

Electron capture negative chemical ionization mass spectrometry is used to analyze cannabinoids in biological matrices.

This document describes a GC/MS method using RTL technology that allows for the analysis of 567 of the most common pesticides and endocrine disrupters used worldwide. By utilizing the RTL functionality with the 6890/5973 GC/MSD system, a new database containing mass spectra and compound retention times has been developed. When combined with the compound screening tools in the MSD Productivity ChemStation software, data files can be easily, accurately, and quickly screened for the presence of any of the 567 compounds listed in the database. Additionally, the software features enable the detection of compounds that may usually be masked by others.

The four antiarrhythmic drugs, quinidine, disopyramide, procainamide and its metabolite N-acetylprocainamide are separated by HPLC using gradient analysis on a reversed phase column and UV detection. The method is accurate and reliable, and can be used for the determination of these drugs in biological fluids.

The document describes a drug called Verapamil, which is a calcium antagonist used to treat hypertension, cardiac arrhythmia, and angina pectoris. The drug also shows some prophylactic effects against migraines.

The HPLC method presented here shows the chromatogram of the four common antiasthmatic drugs theophylline, theobromine, caffeine and emprofylline using isocratic analysis on a reversed phase column and UV detection.

This document introduces the detailed steps for analyzing testosterone and its metabolites using HPLC.

This document describes the collection of fractions from an oligonucleotide separation using gel electrophoresis and offline MALDI-TOF analysis. By using the Agilent oligonucleotide kit and Agilent Capillary Electrophoresis system, antisense oligonucleotides can be successfully separated. The Agilent ChemStation software allows for fully automated collection of fractions using signal A as the peak sensor. This method enables further analysis of the separated peaks and determination of the molecular mass.

This article describes the use of high-performance liquid chromatography (HPLC) for the analysis of fat-soluble vitamins. HPLC can separate and detect these compounds at room temperature while blocking oxygen and light. UV-visible diode-array detection provides both qualitative and quantitative data. Electrochemical detection is also a highly sensitive and selective HPLC method. Different food matrices require different extraction procedures, including alkaline hydrolysis, enzymatic hydrolysis, alcoholysis, direct solvent extraction, and supercritical fluid extraction.

Orthogonal methods for protein purification and purity analysis are important to determine with certainty protein identity and purity. The Agilent 1100 Series purification system and the Agilent 2100 bioanalyzer with the Protein 200 Plus LabChip® kit provide an ideal solution that ensures efficient and reliable protein purification and characterization.

This article introduces the Statistical Peak Identification feature in Agilent Technologies' Cerity NDS for Chemical QA/QC, which addresses the complications in traditional peak identification algorithms caused by peak shifts, changes in concentration, and external forces. It provides a more robust quantitation.

This application note describes the methods for measuring protein size and protein expression using the Agilent 2100 Bioanalyzer and Protein 200 LabChip® kit.

This application note describes how to use microbore reversed-phase HPLC to separate peptide mixtures, to characterize proteins or to isolate peptides for further studies.

This application note describes reproducible methods for sample preparation and HPLC analysis of PNAs in soil, barbecued meat and waste water, all with good recovery rates, and detection limits that exceed regulatory requirements.