Agilent Manuals

This document introduces ZORBAX SB-CN column, including that it is the only acid-stable CN column and has unique selectivity.

This file introduces StableBond short-chain bonded-phase columns from Agilent Technologies, which are much more stable than standard packings and ideal alternatives to C- 18 columns for stable reversed-phase method development.

ZORBAX SB-C18 is the most rugged, stable commercial C18 column produced by Agilent Technologies.

This document describes the characteristics and usage of the ZORBAX GF-250 column. The column has a wide separation range and is suitable for separating proteins with different PI and hydrophobicity.

pH control is very important in providing separation stability for polar organic compounds that are basic or acidic. The short-chain SB-CN column is very stable under low pH conditions (e.g. pH 2.5).

This document describes the use of highly purified, less-acidic Type-B silica support (ZORBAX Rx-SIL) in reversed-phase separation of tricyclic antidepressants, which resulted in improved band shape and column efficiency for these basic drugs. Reduced retention for column with Type-B silica is due to lower surface area of its support.

This document describes the high reproducibility of the Agilent ZORBAX Rx-C8 column in the detection of basic drugs and introduces the strict quality control in the production process of the column.

This document introduces the lot-to-lot reproducibility test of Agilent ZORBAX SB-C8 chromatography column products

This document introduces the characteristics of ZORBAX 3.5-µm silica, which offers higher sample load (over 5-µm particles) with comparable column efficiency.

SB-C3 is a shorter-chain bonded phase that is less retentive than SB-C8 but more retentive than a non-sterically protected TMS phase. SB-C3 can be used to decrease solute retention and is more stable than the non-sterically protected TMS phase.

This document highlights the advantages and characteristics of narrow bore and standard bore separation of polypeptides. It mentions that narrow bore columns require only 20% of the mobile phase and 1/3 of the sample volume compared to standard bore columns under sample-limited conditions. It also emphasizes the need to minimize extra-column volume for optimal efficiency when using narrow bore columns.

This article compares the stability test results of ZORBAX Eclipse XDB-C8 and Symmetry-C8. The ZORBAX particle technology results in an extremely strong and dense silica support that dissolves more slowly at higher pH than xerogel particles used in Symmetry column packings. The extra dense bonding of ZORBAX XDB-C8 helps to shield the silica support at intermediate pH and decreases loss of chromatographic performance compared to Symmetry C8. By combining dense, durable particles, extra-dense bonding, and double endcapping, ZORBAX Eclipse packings (e.g., XDB-C8) have increased lifetime compared to Symmetry columns.

The use of a column packing having reduced particle size can improve efficiency of a separation or, alternatively, increase loading capacity.

This document compares the use of micro and regular flow cells with ZORBAX 300SB-C8 (3.5 µm) column for peptide and protein analysis. It highlights the reduction of analysis time without compromising efficiency. By reducing the flow cell size, the effects of extra-column volume are minimized with small particle-size packing.

This article discusses the effect of Rx silica, eXtra-Dense Bonding, and double end capping on peak shape, as well as the improved chromatographic performance of Eclipse XDB column packings for basic molecules (P and A) at neutral pH. By reducing the interaction of basic molecules with the packing surface, good peak shape is achieved. ZORBAX XDB-C8 exhibits better peak shape than Symmetry C8, even for the strongly basic molecule amitriptyline.