Agilent Manuals

CHIRALPAK QN-AX and QD-AX are anion exchanger chiral stationary phases providing specific enantioselectivity for acidic compounds. In this Application Note, we demonstrate that the application of these columns can be extended to supercritical fluid chromatography (SFC) for enantiomer separation of acidic compounds. Using the advantages of the advanced technology of the Agilent 1260 Infinity Analytical SFC system, we were able to assess the influence of a series of parameters such as the mobile phase additives and the flow rate to gain insight into the general approaches for method development and optimization.

This document introduces the Agilent developed methods for the rapid, sensitive and robust detection of phthalates in food.

This document presents a method for SAMHSA-compliant LC/MS/MS analysis using Agilent Bond Elut Plexa PCX and Agilent Poroshell 120. The method meets SAMHSA guidelines and provides measurement of linearity, limit of detection (LOD), accuracy and precision, matrix effects, extraction recovery, and overall process efficiency. The method utilizes premier Agilent products including Agilent Bond Elut Plexa PCX mixed-mode polymeric SPE sorbent, Agilent Poroshell 120 EC-C18 2.7 µm superficially porous LC column, Agilent 1200 Infinity LC system, and Agilent 6460 Triple Quadrupole LC/MS system.

Agilent Technologies, Inc. has released a new SAMHSA method for the LC/MS/MS analysis of phencyclidine in urine. The method is simple and easy to use, does not require derivatization, and exhibits good performance in terms of a wide range, high accuracy, high recovery, and overall process efficiency.

This document presents a method for SAMHSA-compliant LC/MS/MS analysis of opiates (morphine and codeine) in urine using Agilent Bond Elut Plexa PCX and Agilent Poroshell 120. The method meets the latest SAMHSA guidelines and demonstrates linearity, limit of detection (LOD), accuracy and precision, as well as measurement of matrix effects, extraction recovery, and overall process efficiency. The document also introduces the Agilent products used and their features.

This document describes the SAMHSA-compliant LC/MS/MS analysis of benzoylecgonine in urine using Agilent Bond Elut Plexa PCX and Agilent Poroshell 120. The method's linearity, limit of detection (LOD), accuracy, and precision are demonstrated, as well as measurement of matrix effects, extraction recovery, and overall process efficiency.

This article presents a SAMHSA-compliant LC/MS/MS analysis method for the detection of 6-acetylmorphine in urine. The method utilizes Agilent products such as Agilent Bond Elut Plexa PCX and Agilent Poroshell 120, and demonstrates simplification, linearity, limit of detection (LOD), accuracy, and precision.

This application note describes a method for achieving highly resolved ultrafast separations of reduced and alkylated monoclonal antibodies using an Agilent ZORBAX sub-2 µm Rapid Resolution High Definition (RRHD) 300SB-C8 reversed-phase column under optimized chromatographic conditions. The StableBond C8 phase and RRHD column technology, in combination with robust gradient elution conditions, enabled efficient, fast, and effective separation of light chain and two heavy chain monoclonal antibody variants in under 4 minutes. Additionally, alternate mobile phase compositions were employed, making separations flexible for ultra fast LC/MS analysis. At elevated operating pressures and temperature, the stable C8 phase and rugged column packing technology delivered highly reproducible separations and delivered excellent chromatographic run-to-run results.

This application note shows the possibilities and limitations in fast analysis of saturated and unsaturated C1 to C3 hydrocarbons using an Agilent 490 Micro GC. The chromatograms and results outline the similarities and differences when using a PoraPLOT U and a PoraPLOT Q column channels.

This study presents a robust reverse phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of 10 synthetic colorants in sweets. Separation and quantification were achieved using an Agilent Poroshell EC-C18 column and an Agilent 1260 Infinity LC System. The robustness of the method was established through partial validation. The suitability of this method for quantifying artificial colorants from a food matrix was demonstrated by analyzing color additives in sweets. Additionally, the HPLC method was successfully transferred to a short Ultra High Pressure Liquid Chromatographic (UHPLC) method using an Agilent 1290 Infinity LC System for faster analysis without compromising resolution. The Agilent 1290 Infinity Diode Array Detector (DAD) was used to select various wavelengths for quantifying different colorants at their absorbance maxima. The limits of detection (LOD), limits of quantification (LOQ), precision, accuracy, and linearity of each colorant were established using both methods. Incorporating the Agilent 7696A Sample Prep WorkBench into the analytical workflow facilitated sample preparation for LOD, LOQ, and linearity studies.

This application note describes the methods used to implement batch sample preparation using the Agilent 7696A Sample Prep WorkBench, which can save time and resources.

This study describes the successful cleanup of alkaloids (hydrastine and berberine) in goldenseal using the Agilent Bond Elut Plexa solid phase extraction sorbent. HPLC separation was achieved in less than 6 minutes using an Agilent RRHD ZORBAX Eclipse Plus C18 column (4.6 x 75 mm, 3.5 µm) at 35 °C with a gradient elution of 0.1% phosphoric acid/methanol mobile phase. The calibration curves of both alkaloids showed a good correlation coefficient of 0.9994. The recoveries of hydrastine ranged from 76-102% while those of berberine ranged from 99-104% with a relative standard deviation (RSD) % of less than 5.

SurePrint G3 Human Comparative Genomic Hybridization (CGH) platform has been extended to include a set of Single Nucleotide Polymorphism (SNP) probes on the same microarray. This allows for the simultaneous, high-resolution detection of copy number and copy-neutral variations.

This document describes the separation of recombinant human erythropoietin (rEPO) using Agilent Bio SEC-3. The method utilizes Agilent Bio SEC columns, which are designed to improve separation efficiency and stability by reducing secondary interactions. The columns are optimized for use with organic modifier denaturants and low salt concentrations, making them ideal for quality control in protein primary structure analysis.