Agilent Manuals

The document introduces the use of Gel-Filtration standards and the performance testing of ZORBAX GF-250 columns. The ZORBAX GF-250 column can effectively separate the components of the Bio-Rad® gel-filtration standard between thyroglobulin (670 KDa) and Vitamin B12 (1.35 KDa). The document also discusses the application and results of using gel-filtration test mixtures to test ZORBAX GF-250 columns.

This Application Note describes how to use the Agilent 2100 bioanalyzer to detect mutations in the K-ras and P16 genes.

This document describes the use of mass spectrometry to identify unknown compounds in the pharmaceutical industry. By using a time-of-flight mass spectrometer with accurate mass capabilities, it is possible to determine the structure and composition of unknown compounds more accurately. This is important for the identification of unknown compounds during synthesis and manufacturing processes in the pharmaceutical industry.

The document introduces the advantages of ZORBAX Poroshell packings in the HPLC separation of large molecules such as proteins. This surface porous particle can make the separation faster. At high pH, peptides and small proteins can also be separated by using Extend-C18-bonded Poroshell particles. The high pH may be desirable for improving selectivity, solubility, ease of downstream processing, or to change signal-to-noise and charge-state in LC/MS. By extending the gradient time, it is possible to achieve even higher resolution in typical run times of 10 to 20 min.

This document introduces the method of using ZORBAX 300SB analytical column for protein separation. By optimizing the method, a quick and easy scale-up from analytical to semi-preparative HPLC can be achieved.

ZORBAX Poroshell 300Extend-C18 is a high-pH stable column for LC/MS applications that can improve signal-to-noise ratio

The document demonstrates the improved liquid chromatography analysis of complex biological fluids using fine particle columns by comparing two columns of different lengths and particle sizes on the analysis of a tryptic digest of human serum albumin (HSA). The columns provide better resolution and comparable sensitivity to 150-mm columns, but in one-sixth the time.

This document describes the method for using the Agilent 2100 bioanalyzer to perform flow cytometric analysis of a limited number of cells.

This document introduces the application of the Agilent ZORBAX Eclipse XDB-CN column, which can be used to analyze complex mixtures containing both polar and nonpolar components, such as pesticide mixtures.

This study investigated the comparison of USP-L10 and USP-L7 columns for dissolution and assay testing of norethindrone/mestranol tablets. The results showed that the USP-L10 column provided better peak separation for dissolution testing, while the USP-L7 column provided higher recovery for assay testing.

This document introduces the importance of Agilent 2100 bioanalyzer in mRNA sample quality assessment, and provides detailed usage methods and precautions.

The Agilent 1100 Series micro fraction collector is a high-performance solution for processing complex samples in applications such as proteomics, metabolomics, natural product research, and library generation. This Application Note describes the field of application for the Agilent 1100 Series micro fraction collector and discusses the features and software control of this device. In addition, the main modules of the micro fraction collection system are also discussed.

This application note describes the use of the Agilent RNA 6000 Pico assay for the assessment of genomic DNA contamination in total RNA isolated from embryonic kidney cells using laser capture microdissection (LCM). Integrity and relative concentration of total RNA were analyzed using the Agilent 2100 bioanalyzer and the RNA 6000 Pico assay. An unexpected peak between the 18S and 28S ribosomal RNA peaks was observed and unambiguously proven, by DNase digestion and genomic DNA spike-in experiments, to be caused by genomic DNA contamination. A simple on-column DNase digestion method is suggested to eliminate contaminating genomic DNA from total RNA samples obtained by LCM, fluorescent-activated cell sorting (FACS), or manual dissection.

This Application Note describes an improved method for protein identification with two-dimensional nano LC/MS, which utilizes the elution of digest peptides from a strong cation exchange column by an injected salt step gradient of increasing salt concentration with a subsequent reversed phase separation. However, in this approach the ion exchange chromatography does not produce its full performance. To improve the SCX chromatography performance a new method was developed, which works with a semi-continuous pumped salt gradient. This Application Note describes the improved method for two-dimensional nano LC/MS. To show the full performance of the method a complex tryptic digest of the yeast proteome was analyzed. The results obtained with the developed method are compared to the formerly used injection method as well as to an off-line 2D LC method.