Agilent Manuals

A method for simultaneous determination of 11 β2-agonist residues of clebuterol, salbutamol, ractopamine, terbutaline, salmeterol, propranolol, tulobuterol, cimaterol, mabuterol, mapenterol, and zilpaterol in pork has been developed and validated. The analytes are extracted by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) and quantified by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) operating in positive ion multiple reac- tion monitoring (MRM) mode. The method provides a pg/g level of limit of detection (LOD) for all β2-agonists in pork. The dynamic calibration ranges for these compounds are obtained from 0.25 to 5 ng/g. The overall recoveries range from 82% to 105% with RSD values between 1.6% and 8.4%.

This document describes the analysis of USP-467 residual solvents using Agilent J&W FactorFour VF-WAXms GC columns. The article lists the technical parameters of the GC column and experimental conditions used, and provides chromatograms for the identification of 26 residual solvents involved.

This application note describes a method for identifying oxidation sites on a monoclonal antibody (mAb) using an Agilent 1260 Infinity HPLC-Chip/MS system coupled to an Agilent Accurate-Mass 6520 Quadrupole Time-of-Flight (Q-TOF) LC/MS. A monoclonal antibody was oxidized by treatment with hydrogen peroxide to stimulate potential oxidative modifications that may occur during the manufacturing process. The accurate mass measurement of the 6520 Q-TOF LC/MS combined with Agilent MassHunter BioConfirm software facilitated easy identification of the oxidation sites. Peptides containing amino acid residues such as methionine and tryptophan, which are sensitive to oxidation, were easily identified using this approach.

This article describes the method of detergent removal using Agilent Bond Elut OMIX SCX pipette tips. The Bond Elut OMIX SCX pipette tips have good adsorption capacity and flow characteristics, which can quickly and effectively remove detergents.

This document describes a method for the determination of 24 polycyclic aromatic hydrocarbons (PAHs) in drinking water. The analyte is concentrated using an automated solid phase extraction (SPE) method and analyzed by high-performance liquid chromatography (HPLC) with fluorescence detection.

This application note describes a GC/MS method that resolves all EU and EPA PAHs, and their known interferences, with the Select PAH column in a single run. The method uses enhanced column selectivity and an optimized oven program to resolve all PAHs, including those that co-elute with other PAHs.

This article describes the method and conditions for pesticide analysis by GC/ECD using Agilent J&W FactorFour VF-5 Pesticides and VF-1701 Pesticides columns. The method effectively separates EPA 8081 pesticides and provides peak identification data for VF-1701 Pesticides and VF-5 Pesticides columns.

Agilent PX Scanner is a revolutionary product designed specifically for the identification and X-ray diffraction analysis of protein crystals in situ within multiwell crystallization plates. It is a unique, compact, self-contained unit that combines optical and X-ray imaging. The optical imaging component allows the user to visually locate and select objects, which can then be studied by X-ray diffraction using the built-in microfocus X-ray source, optics, and CCD detector. The 2nd generation Agilent PX Scanner is equipped with a significantly brighter Nova X-ray source and a CCD detector with greatly improved sensitivity. These enhancements make it even more effective at screening protein crystals undisturbed in crystallization plates, saving time by increasing throughput and improving resolution.

This document introduces a method for screening 12 plant growth regulators (PGRs) in grape samples using Agilent 6530 accurate-mass Q-TOF LC/MS system with Agilent 1290 Infinity LC. The method shows excellent sensitivity and outstanding mass accuracy.

This Application Note describes the analysis of milk by on-chip electrophoresis with the Agilent Protein 80 kit and the Agilent 2100 Bioanalyzer. Advantages of this method for milk protein analysis in comparison to conventional SDS-PAGE include: • Fast, reproducible and robust all in one method, replacing gel electrophoresis, staining and densitometric analysis • Easy sample comparison by electropherogram overlay for protein assignment within a chip or in between chips • Automated quantitative analysis for individual protein components

Agilent Bond Elut Plexa PCX is a cation exchange solid phase extraction product with hydroxylated surface, which can reduce the interaction between endogenous material in biological samples and the solid phase extraction sorbent, thereby reducing ion suppression and improving sensitivity.

Agilent Bond Elut Plexa polymeric SPE reduces ion suppression for better sensitivity and low LOD and LOQ. Beta blockers are extracted with excellent correlation coefficients, recoveries, and % RSD.

This document describes an effective, sensitive triple quadrupole LC/MS method with a simple, fast and economic sample preparation technique for the quantitation of 12 multi-plant growth regulators (PGR) in grape samples. Plant hormones or phytohormones are chemicals that regulate plant growth. Manmade compounds, or PGRs are used to regulate the growth of cultivated plants, weeds, and in vitro growth plants and plant cells. Synthetic PGRs are also used in various techniques such as cutting, grafting, and micropropogation. PGRs activate cellular responses such as cell death. Some have the ability to adversely affect human cancer cells. Because the European Union has listed these compounds, they are required to be measured.