Agilent Manuals

This note provides a method for improving GC performance in order to perform trace level GC/MS analysis. The note summarizes suggestions for improving GC performance, including using high purity carrier gas, optimizing sample injection and using optimized GC columns.

The analysis of pesticides in water, foods, and soil is important worldwide. This document discusses the changes and regulations in environmental monitoring in the United States, Europe, Asia-Pacific region, and Latin America. It highlights the challenges and limitations of using gas chromatography/mass spectrometry (GC/MS) for pesticide detection, and the reliance on gas chromatography with an electron capture detector (GC/ECD). It also introduces the new 6890/5973 GC/MS system, which offers lower detection limits and can meet the current demands of environmental monitoring.

This document describes the characteristics of gas chromatograph-mass spectrometer, including electronic pressure control, fast GC furnace, etc., which makes gas chromatograph-mass spectrometer have extremely high productivity, and it can realize small volume sample injection and automatic data processing.

The document describes the ability to identify and quantify compounds based on single and dual column analyses. Instruments used include Agilent Technologies 6890 with other Hewlett-Packard instruments and data systems. The document demonstrates the acquisition and analysis of combined GC/MSD and GC/FID data.

This article introduces the method of optimizing determination of C1-C6 impurities in propylene and ethylene using HP-PLOT/Al2O3 columns. Compared with other commercially available alumina PLOT columns, HP-PLOT columns have better selectivity, increased capacity and complete baseline separation. HP-PLOT column analyses were done using the 5890 Series II gas chromatograph with electronic pressure control and a flame ionization detector.

This Application Note demonstrates mass-based purification of a series of synthetic peptides with the Agilent 1100 Series purification system. The modular set-up of the system, a reliable fully-automated delay volume calibration and a comprehensive software package provide a versatile platform for purification tasks up to 100 mL/min. Crude peptides were successfully purified by reverse phase HPLC from less than 1 kDa to more than 10 kDa, covering a pI range from 4 to 13.

This application note describes a method of using ICP-MS to measure surface metal contamination on silicon wafers by combining VPD with ICP-MS.

Agilent 1100 Series high throughput system introduction

A purification strategy for a medicinal chemistry group at Boehringer Ingelheim in Vienna, Austria, using the Agilent 1100 Series purification system was developed. The goal was to develop a simple purification process, which could be easily used by all chemists. Further challenges were to develop a set of pre-defined methods to purify not only simple samples but also problematic samples, such as insoluble samples, polar samples and samples that show poor ionization.

This document describes the nature and location of all electronic data in a ChemStation Plus networked data system, including the add-on modules ChemStore as central database and Security Pack for support of compliance requirements. The document will help system and laboratory managers to understand how electronic results are managed by a ChemStation Plus system and how all related records are linked to a final result. The document will also help system administrators to understand the format of all ChemStation Plus electronic data, the data location and data organization in the database, and the operating system perspective. The ultimate goal of this document is to give every system user an outline of the ChemStation Plus structure, which will allow them to prove data integrity of their results and of the entire system during an audit or investigation. This document should be stored and managed carefully as the information contained herein could be misused to modify or manipulate ChemStation Plus electronic records.

ChemStation is a chemical analysis software developed by Agilent company, including basic software and data management module ChemStore. This software can generate reports, manage data, and perform second analysis on chemical analysis data.

This document describes the method of removing salts from protein samples prior to analysis on the Agilent 2100 bioanalyzer. This can improve the sensitivity and consistency of the analysis.

Glycoprotein sizing on the Agilent 2100 bioanalyzer

Differential proteome analysis: Two-dimensional nano LC/MS of E. coli proteome grown on different carbon sources