Agilent Manuals

This Application Note describes purification of poly- and monoclonal antibodies from different media backgrounds constructed to mimic those encountered during antibody screening, production, and scale-up in commercial settings.

Scalability of Agilent Columns Across HPLC and UHPLC Instruments

This application note describes a method for the rapid separation of recombinant human erythropoietin using the Agilent ZORBAX 300SB-C18 1.8 µm Rapid Resolution High Definition (RRHD) column. This method has increased sensitivity, reduced separation time and improved run-to-run reproducibility, which is in great demand by biotechnology and pharmaceutical companies. The C18 StableBond coating technology provides increased sensitivity, exceptional pH, and thermal stability. The use of a 1.8-µm column designed for UHPLC systems significantly reduces analysis time, critical for increasing the efficiency of QC for protein primary structure analysis. The data presented here focus on the fast separation of various recombinant human erythropoietin isoforms. Like many other biomolecules, recombinant EPO protein exhibits heterogeneity due to modifications that occur during manufacturing. Methods are optimized for flow rates, gradient and reproducibility under acidic conditions that contain trifluoroacetic acid.

This document describes the analysis of azo dyes using the Agilent 1290 Infinity LC System and the 60 mm Max-Light high sensitivity flow cell. The performance of the high sensitivity method is investigated by comparing the results with those obtained using a standard flow cell.

This application note presents a method developed using the Agilent 1290 Infinity LC Method Development System and the Agilent Method Scouting Wizard software for the analysis of 20 amines derived from banned azo dyes. Different columns, mobile phases, and temperatures were screened in an automated manner. Initial screening analyses were performed using LC-MS, while method optimization was done with diode-array detection. The performance of the final method was evaluated.

This document describes the method of fast refinery gas analysis using the Agilent 490 Micro GC QUAD. The authors demonstrate the analysis results obtained in about two minutes through experimental results. The document also introduces the features of the equipment and the materials used.

This application note describes an improved microextraction method for sample preparation that can be used to analyze a variety of therapeutic categories of pharmaceuticals on an Agilent Poroshell 120 column. The method is simple, fast, economical, and effective for therapeutic drug monitoring and forensic chemistry.

This document describes how a pharmaceutical company maximizes efficiency in early drug discovery by using Agilent Analytical Studio Reviewer (ASR) for high-throughput LC/MS analyses to determine the correct compounds. The company is one of the largest pharmaceutical companies in Japan, with a focus on developing drugs in the therapeutic categories of urology, immunology and infectious diseases, oncology, neuroscience, diabetes mellitus complications, and metabolic diseases.

This study demonstrates the use of Agilent’s new MS/MS spectral metabolomics library to elucidate the identification of several amino acids that are important in malaria metabolism.

This document introduces the Agilent TOP-RNA high-performance RNA oligonucleotide purification technology

This data sheet provides detailed information about the Agilent AMMP-6220 6-20 GHz low noise amplifier, including product features, applications, and description.

This document describes the method of high performance DNA oligonucleotide purification using Agilent TOP-DNA. Synthetic oligonucleotides have become readily available due to solid phase synthesis, but there are some limitations such as the need for high coupling yields and difficulties in purification. Agilent has developed a purification method specifically designed for DNA oligomers up to 150 bases.

This document introduces the application of Agilent RapidFire/MS systems for high-throughput in vitro ADME analysis. The authors emphasize the importance of analyzing cytochrome P450 (CYP) inhibition and the need to evaluate a larger number of samples. They propose a fast, efficient, and cost-effective method for analysis.