Metronidazole: Updating USP Methods Application Note

About this manual

The document discusses an application for the pharmaceutical drug Metronidazole, which is used for antibacterial and antiprotozoal treatment. It highlights the benefits of updating the USP 23 / NF 18 method by using modern silica packing columns from Agilent Technologies instead of traditional ZORBAX C8 columns.

The text compares three specific column configurations: the standard ZORBAX C8, the Eclipse XDB-C8, and the Eclipse XDB-C8 Rapid Resolution. It notes that for acidic compounds like Metronidazole, improved tailing factors and peak shape are best achieved on the ZORBAX Eclipse XDB-C8, while higher efficiency is gained using the Rapid Resolution columns.

What's inside

• Application
• Pharmaceutical
• ZORBAX C8
• Eclipse XDB-C8
• Rapid Resolution
• Mobile Phase
• Detection

Frequently asked questions

What is the stated use of Metronidazole?

Metronidazole is used for antibacterial and antiprotozoal treatment.

What is the required column configuration for the USP 23 / NF 18 method?

The method requires an L7 column with a configuration of 150 x 4.6 mm.

What are the mobile phase ratio and flow rate for the described conditions?

The mobile phase is an 80:20 ratio of Water:Methanol at a flow rate of 1 mL/min.