Agilent Manuals

This Application Note describes the performance comparison and benchmark of the Agilent 2200 TapeStation system with the Agilent 2100 Bioanalyzer system, the industry standard for RNA quality control. The 2200 TapeStation system performs automated analysis of up to 96 samples with approximate analysis time of 1 minute per sample. This Application Note demonstrates the high correlation between RINe generated by the 2200 TapeStation system with the RIN quality assessment of the 2100 Bioanalyzer system. The high reproducibility of RINe achieved with the 2200 TapeStation system in RNA quality assessment is also shown. The 2200 TapeStation system is, therefore, a reliable system for RNA quality control.

This application note introduces the Chinese Pharmacopoeia 2010 edition limit requirements for heavy metals and describes the application of Agilent Technologies 's microwave plasma atomic emission spectrometry (MP-AES) in the determination of heavy metal content in Chinese herbal medicines.

This study focuses on the rapid screening of an entire hippocampus section of mouse brain for the study of Alzheimer’s disease. The aim was to investigate whether there was a correlation between the tissue’s chemical composition and protein secondary structure with tissue morphology, in order to provide insight into the disease process. Agilent’s 670 and 620 FTIR imaging analysis, combined with a chemical imaging microscope and FTIR spectrometer, were used for this research.

This document mainly introduces Agilent 8800 triple quadrupole ICP-MS innovative reaction cell technology, which can effectively eliminate oxide polyatomic ion interference

This application note describes an ultrafast SPE/MS/MS method, using the Agilent RapidFire High-Throughput Mass Spectrometry System, with a sample analysis time of <10 seconds per sample, for the analysis of levetiracetam in human serum. The Agilent RapidFire/MS System exhibited comparable accuracy, precision, linearity, and sensitivity to LC/MS/MS, but with a sample analysis cycle time 10-fold faster than LC/MS/MS.

To ensure food safety and safeguard human health, the characterization of the elemental composition of a wide range of food types is required. The possibility of measuring all the required elements with a single configuration using the Agilent 7700x ICP-MS is investigated. The determination of trace and major elements simultaneously is possible due to the 9 orders of magnitude provided by the detector. Collision cell technology with a single cell gas, helium, is used to eliminate interferences generated by the plasma and the sample matrix. The use of a discrete sampling system to increase sample throughput is also evaluated.

Mercury is one of the more toxic elements to living organisms. It is encountered at relatively low concentrations in the environment but with its amplification through the food chain, the final concentration in some foods can be relatively high. In addition, its toxicity is not only linked to its total concentration but is also dependent on the species. Mercury speciation in food analysis is necessary to fully estimate the toxic potential of this element to humans. Due to the low detection limits required for the analysis of Hg species, GC-ICP-MS has been the preferred method. However, with the sensitivity improvements in modern ICP-MS systems, the use of HPLC has become practical for the separation. Coupling HPLC and ICP-MS is particularly attractive due to the simple and fast connection between the techniques. Furthermore, in contrast to a GC analysis, sample extracts are directly injected into the system and no species derivatization is required, greatly simplifying the sample preparation and reducing the cost of the total analytical procedure.

This application note describes a method using high resolution accurate mass (HRAM) LC/MS for the quantitation of cystine and related metabolites in white blood cells. Excellent assay performance was achieved for cystine quantitation in white blood cells (WBCs) using the ultra-high resolving power and mass accuracy of an Agilent 6530 Accurate-Mass Q-TOF LC/MS System. Further, related metabolites were successfully identified and quantitatively profiled. The HRAM LC/MS data acquired in this study can be retrospectively analyzed to search for more metabolites and biomarkers without sample re-injection.

This application note describes a rapid method for detecting benzoylecgonine (BZE), the major metabolite of cocaine, in urine using the Agilent RapidFire High-throughput Mass Spectrometry System. Results were achieved with much faster sample cycle times and similar analytical output compared to GC/MS or LC/MS assays.

A method for simultaneous determination of malachite green and crystal violet, and their metabolites leuco-malachite green and leuco-crystal violet in fish, was developed and validated. The analytes were extracted by solid phase extraction and quantified by liquid chromatography coupled to electrospray ionization tandem mass spectrometry operating in positive ion multiple reaction monitoring mode. The method provided a 0.5 ng/g limit of quantification for all these compounds in fish. The dynamic calibration ranges for these compounds were obtained from 0.5 to 100 ng/g. The overall recoveries ranged from 96 to 109% with RSD values between 1.7 and 4.5%.

This document introduces the method of optimizing the method parameters of detecting steroids in wastewater using Agilent 6490 Triple Quadrupole LC/MS System with iFunnel Technology, resulting in a sensitivity as low as sub-1 ng/L (1 part per trillion).

This article describes an LC/MS/MS method for the determination of buprenorphine and norbuprenorphine in whole blood using an Agilent Bond Elut Plexa PCX and an Agilent Poroshell 120 column

This Application Note shows that unwanted retention and peak tailing of adenosine triphosphate can be completely prevented when using the Agilent 1260 Infi nity Bio-inert Quaternary LC System. Due to the iron and steel-free design of the Agilent 1260 Infi nity Bio-inert Quaternary LC System, phosphate compounds can be analyzed without any issues regarding the formation of phosphate-iron complexes found with stainless steel systems. Even in the presence of high organic mobile phases, good peak shapes were observed without peak tailing or area reduction.

This application note demonstrates sensitive and reproducible HPLC analysis of N-linked glycans of human immunoglobulin G using the Agilent 1260 Infinity Bio-inert Quaternary LC System with fluorescence detection. The precision of retention times and areas was determined for analysis of a N-linked human IgG glycan library. In addition, good linearity in a range from 0.008 to 1 pmol was determined for a mix of five glycan standards (Man5, G2, G2S1, G2FS1, and G2S2) with low limits of detection and quantification. The Agilent 1260 Infinity Bio-inert Quaternary LC System with fluorescence detection is an optimal system for sensitive and reproducible analysis of 2-AB derivatized N-glycans released from human immunoglobulin G.