This document describes the rapid analysis of dexamethasone using the USP method. By choosing a ZORBAX Rapid-Resolution column configuration, analysis time is reduced by 70% and solvent consumption is subsequently reduced. Improved performance can be achieved by selecting an alternative column configuration.
The document presents an application note detailing a High-Performance Liquid Chromatography (HPLC) method for the pharmaceutical analysis of Dexamethasone. It highlights the use of Agilent ZORBAX SB-C8 columns to reduce analysis time by 70% and solvent consumption compared to standard methods.
The method uses an isocratic mobile phase consisting of water and acetonitrile (ACN) with 30% B.
The procedure requires an injection of 10 µL and a flow rate of 2 mL/min.
Detection is performed using UV at a wavelength of 254 nm.