Agilent Manuals

This application note describes how to isolate active natural products from red clover using the Agilent 1100 Series purification systems.

This Application Note describes how an evaporative light scattering detector (ELSD) can be used with the Agilent 1100 Series purification system for peak-based fraction collection. The system setup is described, and an example is shown of how an ELSD can be used with the Agilent 1100 Series purification system for peak-based fraction collection.

This document describes the analysis method for ergosterol, a pro-vitamin of Vitamin D2, in soil extracts. Ergocalciferol (Vitamin D2) is produced by the ultraviolet irradiation of ergosterol in plants, fungi, and yeasts. The use of Agilent ZORBAX Rapid-Resolution Rx-C18 columns enables fast analysis of soil extracts with good peak shape and retention.

This document describes the method for catecholamine analysis using Agilent ZORBAX SB-C18 column

This document introduces a high-efficiency method for separating nucleosides using reversed-phase separation with protected-bonded-phase columns.

Significant differences in selectivity can be achieved by changing the ZORBAX StableBond phase. Note selectivity and resolution differences for peptides S1 and S2. Peptides S1-S5 are decapeptides that differ in amino acids at two locations, causing only slight differences in hydrophobicity.

This document describes how to improve separation by taking advantage of different bonded-phase chemistries. When using StableBond sterically protected phases, both long-chain and short-chain bonded phases are stable.

This document introduces the effect of column aging with acidic mobile phase on ZORBAX 300SB-C8 (4.6 x 150 mm) (Agilent P/N: 883995-906) column.

This document introduces the separation results of Agilent Technologies' SB-C18, SB-C8, SB-C3, and SB-CN columns at 0-70% B in 36 minutes, and compares them with the separation results on 300Å StableBond columns.

Using elevated temperatures for the reverse-phase separation of peptides and proteins can increase column efficiency, change selectivity, and improve peak shape.

The document discusses the effect of bonded-phase on the resolution and retention of polypeptides. It highlights that differences in bonded-phase selectivity can significantly improve the resolution in reversed-phase analysis of peptides and proteins. The separation between peaks 2 and 3, peaks 5 and 6, and peaks 8 and 9 illustrate these selectivity changes. Additionally, it mentions that temperature can also be used to achieve desired separations. The steric protection of ZORBAX SB packings makes them well suited for chromatography at very low pH.

This document describes how to use small sample injection volumes in size exclusion chromatography