Agilent Manuals

This application note demonstrates the applicability of SFC for typical polymer additives using the Agilent 1260 Infinity Analytical SFC System in combination with an Agilent 6130 Single Quadrupole LC/MS System.

This application note demonstrates the use of Agilent's Mx3005P QPCR System and the resDNASEQ Quantitative CHO DNA Kit (Life Technologies) for the detection of residual CHO host cell DNA. This method produces more specific, more sensitive, and faster results than traditional blot hybridization methods.

Fast separations of intact and reduced monoclonal antibodies (mAbs) were achieved with the use of an Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300 diphenyl reversed-phase column and optimized chromatographic conditions. The unique diphenyl phase and robust rapid resolution high definition column technology, in combination with optimized gradient conditions, provided ultra fast separations and delivered excellent peak shapes demonstrating utility for high throughput mAb charac terizations.

This document introduces the analysis methods of low molecular weight resins from Agilent Technologies, Inc., including molecular weight distribution, composition, crystallinity and aggregation.

This document introduces how to quickly identify o-rings, seals and gaskets using the handheld Agilent 4100 ExoScan FTIR analyzer.

This application note describes the use of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation approach described in the European Committee (EN) for extraction and cleanup of 17 GC-amenable multiple pesticide class residues in apple. The method involves initial extraction in an aqueous/acetonitrile system, an extraction/partitioning step after the addition of salt, and a cleanup step using dispersive solid phase extraction (dispersive SPE). The two different dispersive SPE cleanup approaches (1 mL and 6 mL aliquot volumes) were evaluated simultaneously after sample extraction. The target pesticides in the apple extracts were then analyzed by gas chromatography/mass spectrometry (GC/MS) operating in selective ion monitoring (SIM) mode. The method was validated in terms of recovery and reproducibility. The limit of quantitation (LOQ) for pesticides in apple is 10 ng/g. This application employed Agilent's Bond Elut QuEChERS kit and produced results well below the maximum residue limits (MRLs) for all the pesticides screened. The spiked levels for the recovery experiments were 10, 50, and 200 ng/g. Recoveries ranged between 68 and 112% (86.0% on average), with RSD below 15% (4.7% on average).

Analysis of Pesticide Residues in Spinach Using Agilent Bond Elut QuEChERS AOAC Kits by GC/MS

This paper presents an analytical method for the determination of 11 quinolone antibiotic residues in bovine liver. The method involves a rapid and efficient pretreatment using Agilent Bond Elut QuEChERS kits, followed by extraction and partitioning, and dispersive solid phase extraction for cleanup. The compounds are then determined using LC-ESI-MS-MS in positive ion MRM mode. The method demonstrates good accuracy and precision, with a limit of quantification significantly lower than the maximum residue limit for quinolones in food producing animals.

This application note describes a method for determining fatty acid methyl esters (FAMEs) in salmon oil using automated sample preparation. The method involves the conversion of salmon oil to FAMEs, which are then measured by gas chromatography. The method has good repeatability and saves time.

This Application Note describes the separation of four proteins by anion exchange chromatography using four different high salt-containing elution buffers (up to 2 M) for linear and step gradients.

This application note describes a method for rapidly determining the PE:PP ratio in blends using the Agilent Cary 630 FTIR spectrometer.

This application note describes the development of a sample extraction, cleanup, and enrichment method with an Agilent Bond Elut Plexa cartridge and a separation method using an Agilent Poroshell 120 column, with quantification by HPLC. The method is simple and effective for the extraction, enrichment, and analysis of multiple phenol compounds in drinking water.

This application note presents the performance of Agilent Bond Elut Plexa multi-compound analysis system for the detection of six systemic antifungal drugs in human plasma. The system includes Agilent Bond Elut Plexa polymeric sorbent, Agilent Pursuit XRsUltra 2.8 Diphenyl column and Agilent 1260 Infinity LC Triple Quadrupole LC/MS. Agilent Bond Elut Plexa delivered extremely clean samples, which was demonstrated by detection down to pg levels. All compounds showed near 100% recovery and a single digit % RSD. Superb linearity was achieved in the calibration curves with R2 ¡ 0.995 for all compounds.

This study investigates the blind added multipesticides in cherry tomato and cucumber using Agilent Bond Elut QuEChERS extraction kits and Agilent 5975T LTM GC/MSD. The method allows quick onsite pesticides diagnostics with the transportable Agilent 5975T LTM GC/MSD using Agilent Technologies DRS software, RTL pesticides library, and fast Bond Elut QuEChERS sample preparation. The instrument provides the same excellent performance as previous Agilent mass spectrometers.

Analysis of Pesticide Residues in Apples using Agilent Bond Elut QuEChERS AOAC Kit by LC/MS/MS Detection