Agilent Manuals

A method has been developed on the Agilent 7000 GC Triple Quadrupole GC/MS/MS system for the analysis of foods for 20 chemicals that may be present in printing inks applied to the external surface of cartonboard packaging material. These substances may diffuse through the cartonboard or set-off onto the food contact surface and then migrate into food. The method described is suitable for the reliable determination of ink substances in packed foods at single-figure parts-per-billion (ppb, µg/kg) levels.

This application note introduces a method to enable DBS analysis of basic drugs on Agilent Bond Elut Dried Matrix Spotting (DMS) cards, which is achieved by optimizing desorption conditions.

This document introduces how to detect synthetic cannabinoids in oral fluid

An Agilent 1290 Infinity LC System is optimized for the lowest possible extra-column volume, to ensure minimal band broadening and optimal results from a small dimension, Agilent ZORBAX RRHD Eclipse Plus C18, 2.1 × 50 mm, 1.8 µm column. Improvements to isocratic and gradient analyses are evident, with the early eluting peaks from the isocratic analysis showing the greatest improvement of up to a 58% increase in efficiency (k’ = 1.6). Gradient analyses are improved by 24 to 32% with respect to conditional peak capacity (k’ = 1.3 to 5.3). Additionally, there is no strong correlation between flow rate and the effect of extra-column volume, with respect to column performance for isocratic and gradient analyses.

This application note describes a rigorous QC column testing approach that employs active tests probes to verify the inertness performance of modern GC capillary columns.

This application note describes an analytical method for determination of carbendazim and 3 other fungicide residues in orange juice with easy sample preparation and LC/MS/MS detection. Developed for the 3 most important systemic fungicides, carbendazim, thiabendazole, and imazalil with its metabolite, this method can be easily expanded to include a broader range of pesticides. A simple and rapid extraction procedure provides clean extracts that without any preconcentration allow for detection of analytes at concentrations below 2 ppb. Quantification was performed using triphenyl phosphate as an internal standard. Excellent accuracy (% recovery) and precision (% CV) data were obtained for three concentration levels: 10, 50, and 250 ppb of each analyte.

This document describes the advantages and disadvantages of copper and molybdenum sources on a dual source SuperNova diffractometer, and compares the two sources in terms of data collection and data processing.

This application note uses Agilent ZORBAX RRHD columns with UHPLC/MS to compare Morphine’s HILIC and RPLC. Both methods use acetonitrile/ammonium acetate buffer mobile phase for isocratic elution. The results show that HILIC mode allows for more sensitive MS detection due to more efficient spraying and desolvation in the ESI-MS source, as a result of the volatile acetonitrile rich mobile phase used.

This application note describes the optimization of a rapid LC/MS/MS method for vitamin B related compounds (4-aminobenzoic acid, nicotinamide, riboflavin, and nicotinic acid) on an Agilent ZORBAX Rapid Resolution High Definition HILIC Plus column using an Agilent 1290 Infinity LC System with an Agilent 6410A Triple Quadrupole Mass Spectrometer. The method uses isocratic elution with an acetonitrile rich mobile phase and an ammonium acetate buffer. All analytes have good peak shape with this hydrophilic interaction chromatography (HILIC) application. The method is then used to demonstrate the importance of choosing an appropriate injection solvent when performing LC analyses in HILIC mode. Various injection solvents are investigated, including water, acetonitrile, methanol, and combinations. Of the solvents explored, it was determined that pure acetonitrile yields the best retention and peak shape for all compounds, with water being the worst solvent for analytical performance and peak shape.

Solid-phase extraction (SPE) coupled with high-performance liquid chromatography (HPLC) was optimized for extraction and quantification of two fungicides (carbendazim and thiabendazole) in apple juice. Results indicate that SPE using Agilent Bond Elut SCX (60 mg, 3 mL) and HPLC using an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm × 100 mm, 3.5 µm) is an excellent combination for extraction and analysis of these compounds. Recoveries ranged from 92.1 to 99.4 percent with RSDs below 5 percent and limits of detection of 4 µg/kg.

This application note describes a method for characterizing olive oil using Agilent GC/Q-TOF MS and Mass Profiler Professional software. The method can detect a large number of compounds in olive oil and perform quantitative analysis.

This applications note describes using VnmrJ 3 Software to set up advanced experiments to resolve overlapping homonuclear resonances. These tools allow even novice users to gain critical information about their research samples, using the most advanced NMR experiments available.

This application note demonstrates the concordance in the results achieved with identical oligonucleotide probes on two versions of Agilent miRNA microarrays [(AMADID 021827, release 12.0) and (AMADID 031181, release 16.0)], based on different revisions of the miRBase database and provided on different microarray formats. Since probes for a given microarray will remain constant for subsequent versions of Agilent SurePrint G3 Human miRNA microarrays, barring any miRBase sequence changes, it is necessary to confirm that the TotalGeneSignal achieved is consistent. The data show Agilent’s strength in probe reproducibility, a crucial factor for identifying miRNA of differential expression.

Gene expression analysis is currently carried out using three methods that provide complementary data: microarrays, RNA-Seq, and RT-qPCR. Microarrays provide comprehensive whole-genome coverage in single experiments, with rapid data acquisition and results at a low price per sample.