Agilent Manuals

This document describes a method for determining naproxen levels in serum using a Zorbax SPE C18 cartridge. The method uses solid phase extraction to significantly reduce the levels of interfering substances. The target analyte is then quantified by reversed-phase HPLC.

This document describes a method for extracting quinine from serum using a Zorbax SPE C18 column. The method is illustrated with a flow chart, a list of reagents, a sample preparation method, chromatographic conditions, and sample results.

The document describes a solid phase extraction method for a pharmaceutical compound called Ranitidine. This compound is used for the treatment and prevention of ulcers and for the relief of heartburn. By using a Zorbax SPE C18 cartridge, levels of interfering substances in the bloodstream can be significantly reduced, allowing for easy quantitation of the analyte of interest using reversed-phase HPLC.

The document describes the significant reduction of matrix compounds in plasma by using a Zorbax SPE C18 cartridge, facilitating the quantification of testosterone-like compounds by reversed-phase HPLC. The document also provides methods for solid phase extraction and sample preparation.

This document introduces the Agilent 2100 bioanalyzer and LabChip DNA analysis technology, which has the advantages of high speed, high precision, and ease of use

This document introduces a method for optimizing cRNA fragmentation using the Agilent 2100 bioanalyzer for microarray experiments.

This document describes a method for purifying human erythropoietin (EPO) from formulated drugs using reversed-phase chromatography. This method allows EPO to be purified to homogeneity for further analysis by peptide mapping, mass spectrometry and protein sequencing.

Absolute quantitation with the Agilent 2100 Bioanalyzer and the Protein 200 LabChip® kit. The Protein 200 LabChip® kit allows sizing and quantitation of proteins ranging in size from 14 to 200 kD. Relative quantitation is performed in comparison to the upper marker, used as internal standard in each sample. However, the accuracy of the relative quantitation is affected by the staining efficiency of the protein dye. Absolute quantitation is enabled with the Agilent 2100 bioanalyzer software (revision A.02.01) using the Protein 200 assay in combination with protein standards of known concentrations. Absolute quantitation improves quantitation accuracy by eliminating the differences in staining efficiency among proteins. This Application Note describes how to perform absolute quantitation with the Protein 200 assay and demonstrates the obtained improvement in accuracy.

The document describes the detection of the major metabolite of cocaine in the hair of 2000-year-old Chilean mummies. This is particularly important in relation to Rohypnol® because victims of the drug may not immediately realize they have been violated. However, hair analysis is challenging due to the difficulty in digesting the matrix and the low concentrations of the metabolite. Therefore, the sensitivity must be in the picogram per milligram range. The metabolism of flunitrazepam produces the 7-aminoflunitrazepam metabolite through the reduction of the nitro-group. Flunitrazepam is a benzodiazepine marketed by Hoffman-La Roche under the brand name of Rohypnol®. It is not legally used in the United States, but its abuse has been increasing in the southern and southwestern regions. The relatively low street price and high potency of Rohypnol® are of particular concern.

This Application Note describes results in protein impurity testing and simultaneous molecular mass characterization with the Agilent 1100 Series GPC-SEC analysis system.

The sequencing of oligosaccharides in glycosylated natural products was successfully investigated using liquid chromatography-mass spectrometry with electrospray ion source in negative ion mode.

This application note describes how the DNA 12000 LabChip® kit can be used with the Agilent Technologies 2100 bioanalyzer to size DNA fragments generated by restriction enzyme digestion. Sizing accuracy was found to be greater than 92 % for all samples analyzed. Compari- son of the chip-based separations to those obtained using agarose gel electrophoresis indicates that the DNA 12000 assay accurately sizes fragments over a wider range than can be usually analyzed on a slab gel.