This document describes a method for high-resolution chiral separation of R- and S-Norfluoxetine using Ultron ES-Pepsin. The method achieves excellent separation and extends the column's useful lifetime.
This document describes a method for the high-resolution separation and accurate quantitation of the chiral forms of Norfluoxetine (S- and R-Norfluoxetine). The application highlights the exceptional resolution achieved on the Ultron ES-Pepsin column, which complements the Ultron ES-OVM column to achieve previously impossible separations.
The R- and S- chiral forms of Norfluoxetine are separated with a high resolution of 4.0 under the specified conditions.
The application uses an Ultron ES-Pepsin column with dimensions of 4.6 x 250 mm.
The mobile phase consists of a 6:94 volume ratio of MeOH to 20mM KH2PO4.