Agilent Manuals

Size-exclusion chromatography is a powerful technique for the size-dependent separation of biomolecules. While standard, non-denaturing mobile phases are often desirable, denaturing mobile phases containing guanidine hydrochloride (or SDS), may be used to insure un-aggregated, monomers for proper size determination. As shown below, denaturation shifts molecules toward larger apparent size and smaller retention volume.

The document highlights the advantages of using ZORBAX SB-C18 columns for gradient separations of complex environmental mixtures, demonstrating resolution >1.5 and good peak shapes for all components. The conditions used by Robert Ricker in the environmental analysis application are ZORBAX SB-C18 column, 20% ACN/80% 0.01 M H3PO4 solution, gradient elution from 20% ACN to 45% ACN in 7.5 minutes, followed by gradient elution from 45% ACN to 80% ACN in 2.0 minutes, flow rate of 1.5 mL/min, and temperature of 35°C.

This document describes a method for separating DNPH-derivative aldehydes using reversed-phase chromatography, which can detect very low concentrations of aldehydes.

This Application Note describes four separation strategies for purifying compounds from non-baseline separated peaks.

This document describes the performance comparison of the non-endcapped Agilent ZORBAX SB-C18 column and its doubly endcapped counterpart in the separation of fat-soluble vitamins.

This document introduces the method of separating basic compounds using Agilent ZORBAX Stablebond column, including column conditions, pH effect on separation selectivity.

This document describes the separation of a series of substituted anilines.

The analysis of organic acids is important for both the food and cosmetic industries. Organic acids are naturally found in a wide variety of food products, fruits, and corresponding beverages. They are also used in the cosmetic industry as dermatological agents. Agilent ZORBAX StableBond columns are highly stable at low pH, including the use of mobile phases containing 0.1% trifluoroacetic acid at pH 2.1. ZORBAX StableBond columns provide good peak shapes for acidic compounds.

This document introduces a method for analyzing highly hydrophilic compounds using the Agilent ZORBAX Rx-SIL column and a reversed-phase mobile phase. This method can effectively separate urea and uracil, which are both unretained by reversed-phase columns because they are highly hydrophilic.

This document describes how to use high-performance liquid chromatography (HPSEC) to monitor differences in synthetic polymer production. Specifically, the material uses the ZORBAX Bimodal S column (Agilent P/N: 880957-814) to perform size exclusion separations and uses ultraviolet detection (UV 254 nm) to detect the quality of polymethacrylate materials.

The document describes the features and characteristics of commercial polyethylene, including the requirement of elevated temperature and aggressive solvent systems for high-performance size exclusion chromatography (HPSEC). It also highlights the stability of ZORBAX PSM size exclusion columns with aggressive solvent systems. The document provides detailed analysis conditions and information about the application in polymers. It is authored by Robert Ricker, an application chemist at Agilent Technologies.

This document describes a method for rapid quality testing of uncured epoxy resins. The method utilizes HPSEC instrument with the following conditions: column: DuPont HPLC ZORBAX PSM 60S, 6.2 mm x 25 cm (Agilent P/N: 880957-802); mobile phase: tetrahydrofuran; flow rate: 1.5 mL/min; temperature: 22°C; detector: UV Abs (254 nm); sample: Epon 836.

The document introduces a stable C18 column that can be used for high temperature separation of low molecular weight polymers