This document describes the separation performance of ZORBAX XDB-C8 for Procainamides and metabolites at a pH of 7.0 or higher. It provides good peak shape and resolution, making it suitable for pharmaceutical applications.
The document details a chromatographic method for separating procainamide, n-acetylprocainamide, and n-propionylprocainamide. It highlights that while USP assays often use pH 7.5, the ZORBAX XDB-C8 column provides superior peak shape and resolution at intermediate pH levels (e.g., 7.0) due to its extra-dense bonding and double endcapping which shields active silanols.
The provided method utilizes a mobile phase of 20 mM NaHPO4 (pH 7.0) with acetonitrile, an injection volume of 5 µL, a flow rate of 1.5 mL/min, and a column temperature of 40°C.
The column used in this application has dimensions of 4.6x 150 mm.
The procedure requires an injection of 5pL and a flow rate of 1.5 mL/min.