This document describes the features of ZORBAX StableBond C8, which has a sterically protected bonded phase that allows stable operation at low mobile-phase pH. ZORBAX SB-C8 separates basic compounds with good resolution and peak shape using simple mobile phases. The document also provides the conditions for using ZORBAX Rx/SB-C8 in pharmaceutical applications.
This document presents an application demonstrating the separation of four antihistamines: Pseudoephedrine, Scopolamine, Pyrilamine, and Chlorpheniramine. The analysis utilizes the ZORBAX StableBond C8 column, which features a sterically protected bonded phase allowing for stable operation at low mobile-phase pH.
The chromatographic method employs a simple mobile phase consisting of 10% acetonitrile and 90% 50mM KH2PO4 at pH 2.5. The separation is performed on a 4.6 x 150 mm column at 25°C with a flow rate of 2.0 mL/min and UV detection at 254 nm.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Mobile Phase | 10% ACN, 90% 50mM KH2PO4, pH 2.5 |
| Flow Rate | 2.0 mL/min |
| Detection Wavelength | 254 nm |
| Part Number | 883967-901 / 883975-906 |
The ZORBAX StableBond C8 column is used for the separation.
The mobile phase consists of 10% acetonitrile and 90% 50mM KH2PO4 at pH 2.5.