This document describes the high-resolution separation method and characteristics of nonsteroidal anti-inflammatory drugs, as well as the narrow-bore column configuration suitable for LC/MS applications. The Eclipse XDB-C8 column is capable of separating acidic, basic, and neutral molecules with good peak shape. Specific analysis conditions and the pKa values of the drugs are provided.
This document details an application for the fast and accurate quantitation of nonsteroidal anti-inflammatory drugs (NSAIDs). It highlights the use of a narrow-bore column configuration to achieve high-resolution separation of a mixture containing phenacetin, tolmetin, phenylbutazone, and fenoprofen.
The procedure requires an injection of 2pL at a flow rate of 0.2 mL/min and a temperature of 35°C, with detection at UV(254 nm).