This document describes the separation of cephalosporins on Agilent ZORBAX XDB-C8 column.
This document describes a chromatographic separation method for five cephalosporins (Ceftazidime, Cefotaxime, Cefaclor, and two others) using a ZORBAX XDB-C8 column. The method operates at pH 7.0 to ensure good peak shape by avoiding partially ionized sample components.
The separation utilizes a mobile phase consisting of 85:15 25 mM Na2HPO4 (pH 7.0) and Acetonitrile (ACN). The analysis is performed with a 4.6x 150 mm column at 40°C, with a flow rate of 1 mL/min and UV detection at 254 nm.
| Column Dimensions | 4.6x 150 mm |
|---|---|
| Mobile Phase Ratio | 85:15 |
| Mobile Phase Concentration | 25 mM Na2HPO4 |
| Injection Volume | 5pL |
| Flow Rate | 1 mL/min |
| Detection Wavelength | 254 nm |
Separation is best achieved at pH 7.0 to avoid partially ionized sample components.
The mobile phase is a mixture of 85:15 25 mM Na2HPO4 (pH 7.0) and Acetonitrile (ACN).