The document describes the method and advantages of using ZORBAX Eclipse XDB-C18 for HPLC analysis. By using ZORBAX Eclipse XDB-C18, improved peak shape, higher separation efficiency, greater sensitivity, reduced analysis time, reduced back pressure, and reduced solvent use per analysis can be achieved. The performance of this product surpasses the requirements of USP, with a higher number of theoretical plates and a closer tailing factor to the ideal value.
This document presents an application method for the high-performance liquid chromatography (HPLC) analysis of Pramoxine, a local anesthetic. It compares the performance of the original USP method utilizing older L1 columns against a modern alternative using the Agilent ZORBAX Eclipse XDB-C18 column.
The text highlights that the ZORBAX Eclipse XDB-C18 offers superior efficiency and peak shape compared to traditional 10 µm C18 or L1 columns. Key benefits include greater sensitivity, reduced analysis time, lower back pressure, and shorter solvent usage due to the availability of shorter column lengths (e.g., 150 mm). The document provides specific chromatographic conditions used for this comparison.
| Column Type | ZORBAX Eclipse XDB-C18 |
|---|---|
| Particle Size Options | 3.5 and 5.0 um |
| Column Dimensions (Modern) | 4.6 x 150 mm |
| Flow Rate (Modern Column) | 1 mL / min. |
| Theoretical Plates (N) | 12,200 |
| Mobile Phase Composition | 55% ACN : 45% 50 mM KH2PO4, pH 7.5 |
| Detection Wavelength | 224 nm |
| Column Part Number | 993967-902 |
Using this column offers greater sensitivity, reduced analysis time, reduced back pressure, and shorter length which reduces solvent use per analysis.
The number of theoretical plates is more than 8 times greater on the modern column (12,200 vs. 1500).