The Stratagene XL10‑Gold Chemically Competent Cell manual provides specifications, storage conditions, and detailed transformation protocols.
The XL10‑Gold Chemically Competent Cell is a high‑efficiency strain developed by Stratagene for transforming large plasmids or valuable ligation products. It is supplied as 100 µl vials (CAT# DL1050) with a genotype that removes most restriction systems and endonucleases, enhancing DNA yield and stability.
The manual describes the recommended storage at –80 °C (up to 6 months), the standard heat‑shock transformation procedure (42 °C for 30 s), and media preparation. Precautions such as gentle handling of cells and antibiotic resistance limits are also included. This manual is written in Chinese.
| Transformation efficiency | >2 × 10⁹ cfu/µg DNA |
|---|---|
| Storage condition | -80 °C for 6 months |
| Genotype | Tet R Δ(mcrA)183 Δ(mcrCB‑hsdSMR‑mrr)173 endA1 supE44 thi‑1 recA1 gyrA96 relA1 lac Hte |
| Cell amount per vial | 100 µl |
Heat‑pulse the cells in a 42 °C water bath for 30 seconds.
Store the cells at –80 °C, where they remain viable for up to six months.
The strain is resistant to tetracycline and to chloramphenicol up to 40 µg/ml.
The cells can achieve a transformation efficiency of up to 2 × 10⁹ cfu per µg of DNA.