This is an instruction manual for a product called ViraPort cDNA Library Retroviral Supernatants, which is produced by Agilent. The manual contains information on how to use the product, warranty terms and conditions, and more.
The document is an instruction manual for the ViraPort cDNA Library Retroviral Supernatants (BN #972000-12), produced by Stratagene. It details the materials provided with the kit, such as frozen tissue culture supernatant containing VSV-G pseudotyped retrovirus and lyophilized primers. The manual specifies storage conditions at -80°C for the supernatants and -20°C for primers.
| Virus Titer (Supernatant) | > 2 X 10^5 infectious virus particles |
|---|---|
| Primer Quantity (5' Retro primer) | 2.5 ug |
| Primer Quantity (3' pFB primer) | 2.5 ug |
| Virus Titer (Control Viral Supernatant) | > 10^8 infectious virus particles |
| Filter Size | 0.45 um |
The retroviral supernatant should be stored at -80°C immediately upon arrival.
The lyophilized primers should be stored at -20°C.
Resuspend each primer in 25 ul of TE buffer to a final concentration of 100 ng/ul.