This is the instruction manual for StrataClone Ultra Blunt PCR Cloning Kit
This document is an instruction manual for the Agilent StrataClone Ultra Blunt PCR Cloning Kit (Catalog #240218). It outlines the kit's contents, storage conditions, and required additional materials such as a thermocycler and specific agar plates. The guide explains the technology behind the kit, which combines high-fidelity PCR amplification with a robust topoisomerase-based cloning method to simplify the insertion of blunt-ended PCR products into vectors.
| PCR reactions supported | 40 |
|---|---|
| Cloning reactions supported | 21 |
| Competent Cell transformations supported | 21 |
| Recommended storage for Competent Cells and Control Plasmid | -80°C |
| Recommended storage for other components (before first use) | -80°C |
| Recommended storage for other components (after thawing) | -20°C |
They must be stored at -80°C.
They should be stored at -80°C prior to first use.
A thermocycler, LB-ampicillin or LB-kanamycin agar plates, LB medium, and X-gal.