Brilliant II SYBR® Green QPCR Master Mix INSTRUCTION MANUAL is the manual for Agilent's product. It introduces the product's usage, warranty terms, ordering information, etc.
This document provides instructions for the use of the Brilliant II SYBR Green QPCR Master Mix, a reagent designed for quantitative PCR amplifications using SYBR Green I dye detection. The kit includes a master mix with SureStart Taq DNA polymerase and a passive reference dye, suitable for high-throughput applications on various real-time PCR platforms including Stratagene Mx3000P/Mx4005P and ABI 7900HT systems.
The manual details the materials provided in single and 10-pack kits, storage requirements (–20°C), and necessary additional equipment such as a spectrofluorometric thermal cycler. It outlines pre-protocol considerations regarding primer design to minimize nonspecific amplification, data acquisition methods, and specific PCR cycling programs for two-step protocols.
| Reaction Volume Capacity | Four hundred, 25-μl reactions |
|---|---|
| SYBR Green I Excitation Max | 497 nm |
| SYBR Green I Emission Max | 520 nm |
| Fluorescence Increase upon Binding | Approximately 1000 fold |
Store the 2× master mix at 4°C after thawing.
Once thawed, full activity is guaranteed for 6 months.